Pezeshki Seyed Mohammad Sadegh, Jalali Mohammad Taha, Amin Asnafi Ali, Jaseb Kaveh, Saki Najmaldin
Thalassemia & Hemoglobinopathy Research Center, Health Research Institute.
Hyperlipidemia Research Center, Diabetes Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
J Pediatr Hematol Oncol. 2020 Jan;42(1):e32-e37. doi: 10.1097/MPH.0000000000001543.
Immune thrombocytopenic purpura (ITP) is a bleeding disorder characterized by low platelet counts in peripheral blood, impairment of thrombopoiesis in bone marrow, and risk of mild to severe bleedings. ITP can be seen among both sexes in different ages. Although definitive pathogenesis of this disorder is still ambiguous, some of risk factors for ITP are recognized, including human leukocyte antigens (HLAs).
Our goal was to evaluate the possible association between HLA-B5, 7, 8, 27, and 51 antigens with ITP for the first time. We were hoping to achieve new hypothetical diagnostic/prognostic biomarkers to introduce a new subject for further studies on HLA class I antigens as possible risk factors for ITP.
A total of 37 patients with ITP were included in this study. After confirmation of ITP diagnosis, peripheral blood samples were collected from them. The expression of each of HLA antigens was evaluated by standard lymphocytotoxicity technique.
Compared with other studied antigens, the expression of HLA-B5 and HLA-B51 was more prevalent among our patients. According to the results, 22% of patients were positive for HLA-B5 and HLA-B51. Furthermore, no significant association was found between HLAs expressions with complete blood count parameters.
We conclude that there is an association between HLA-B5 and HLA-B51 with ITP and that they are not likely to be used as diagnostic or prognostic biomarkers. We suggest studying the association between HLA-B antigens and ITP in large-scale studies to determine whether or not there is a significant association.
免疫性血小板减少性紫癜（ITP）是一种出血性疾病，其特征为外周血血小板计数低、骨髓中血小板生成受损以及存在轻度至重度出血风险。ITP可见于不同年龄的男女。尽管该疾病的确切发病机制仍不明确，但已认识到一些ITP的危险因素，包括人类白细胞抗原（HLA）。
我们的目标是首次评估HLA - B5、7、8、27和51抗原与ITP之间的可能关联。我们希望获得新的假设性诊断/预后生物标志物，为进一步研究HLA I类抗原作为ITP可能的危险因素引入新课题。
本研究共纳入37例ITP患者。在确诊ITP后，采集他们的外周血样本。通过标准淋巴细胞毒性技术评估每种HLA抗原的表达。
与其他研究的抗原相比，HLA - B5和HLA - B51在我们的患者中表达更为普遍。根据结果，22%的患者HLA - B5和HLA - B51呈阳性。此外，未发现HLA表达与全血细胞计数参数之间存在显著关联。
我们得出结论，HLA - B5和HLA - B51与ITP之间存在关联，且它们不太可能用作诊断或预后生物标志物。我们建议在大规模研究中研究HLA - B抗原与ITP之间的关联，以确定是否存在显著关联。
