Stura E A, Arevalo J H, Feinstein A, Heap R B, Taussig M J, Wilson I A
Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, California 92037.
Immunology. 1987 Dec;62(4):511-21.
Anti-progesterone monoclonal antibodies are being used for structural studies of antibody-antigen interaction, for their ability to block pregnancy shortly after fertilization, and for hormone immunoassay. A mouse anti-progesterone monoclonal Fab' fragment has been crystallized in its native form and co-crystallized with seven different, but structurally related, steroids. The crystals show interesting preferences in their crystal morphology, depending on the bound steroid ligand. The X-ray crystallographic analysis of this Fab', complexed with a series of related steroid ligands, should reveal details of the chemistry of antibody-antigen union and provide insights into how steroids interact with proteins.
抗孕酮单克隆抗体正被用于抗体 - 抗原相互作用的结构研究、因其在受精后不久阻断妊娠的能力以及激素免疫测定。一种小鼠抗孕酮单克隆Fab'片段已以其天然形式结晶，并与七种不同但结构相关的类固醇共结晶。根据结合的类固醇配体不同，晶体在其晶体形态上表现出有趣的偏好。对这种与一系列相关类固醇配体复合的Fab'进行X射线晶体学分析，应能揭示抗体 - 抗原结合的化学细节，并深入了解类固醇与蛋白质的相互作用方式。
Zotero 插件