M315独特型表位与其单克隆抗独特型抗体Fab片段之间复合物的结晶。

Crystallization of the complexes between M315 idiotope and its monoclonal anti-idiotopic antibody Fab fragment.

作者信息

Matsuura Y, Inaka K, Kusunoki M, Katsube Y, Sakato N, Fujio H

机构信息

Institute for Protein Research, Osaka University, Japan.

出版信息

Biochim Biophys Acta. 1987 Dec 18;916(3):524-6. doi: 10.1016/0167-4838(87)90199-3.

DOI:10.1016/0167-4838(87)90199-3

Abstract

The Fab fragment of a monoclonal anti-idiotopic antibody against M315 has been isolated and its complexes with Fv and Fab' fragment of M315 have been crystallized by using poly(ethylene glycol) 6000 or ammonium sulfate. X-ray diffraction photographs showed that the crystal of the complex with Fv diffracts better than that with Fab'. The Fv-complexed crystal was shown to be tetragonal I4, with cell dimensions a = 152 A and c = 69 A, and to contain one complex molecule of about 75,000 molecular weight in the crystallographic asymmetric unit.

摘要

一种针对M315的单克隆抗独特型抗体的Fab片段已被分离出来，并且其与M315的Fv和Fab'片段的复合物已通过使用聚乙二醇6000或硫酸铵进行了结晶。X射线衍射照片显示，与Fv形成的复合物晶体比与Fab'形成的复合物晶体衍射效果更好。Fv复合物晶体显示为四方晶系I4，晶胞参数a = 152 Å，c = 69 Å，并且在晶体学不对称单元中包含一个分子量约为75,000的复合物分子。

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