Akimov M U, Zhbanova E V, Makarov V N, Perova I B, Shevyakova L V, Vrzhesinskaya O A, Beketova N A, Kosheleva O V, Bogachuk M N, Rylina E V, Luk'yanchuk I V, Mironov A M
I.V. Michurin Scientific Centre, Michurinsk, Tambov Region, Russia.
LLC «Experimental Centre "M-KONS-1"», Michurinsk, Tambov Region, Russia.
Vopr Pitan. 2019;88(2):64-72. doi: 10.24411/0042-8833-2019-10019. Epub 2019 Mar 13.
One of the current areas in research of fruit and berry crops is the study of their nutritional value and micronutrient composition. Valuable chemical composition of the strawberry determines a wide spectrum of its utilization not only as fresh fruit, but also as a raw material for food industry. Breeding requirements for contemporary strawberry varieties together with yield, large-fruit, important taste and dietary qualities of fruit provide for higher content of biologic active substances. of the research was an integrated assessment of strawberry fruit for content of nutrients and bioactive compounds, as well as mineral elements, and its antioxidant activity. . The objects of investigation were berries of 5 strawberry varieties bred in the I.V. Michurin Scientific Centre (Lakomaya, Prazdnichnaya, Privlekatelnaya, Urozhaynaya CGL, Feyerverk) and 9 foreign varieties (Vima Zanta, Dukat, Zefir, Kama, Maryshka, Red Gauntlet, Festivalnaya romashka, Honey, Elsanta) which are promising for cultivation in the Black Soil Region of Russia. The content of soluble solids was determined by the refractometric method, sugars - by Bertrand's method, pectins - by the volumetric method, anthocyanins - by pH-differential spectrophotometry. Total content of antioxidants was defined by amperometric method with recalculation per quercetin. Determination of vitamin B1 was carried out using the fluorometric thiochrome method, vitamin B2 - by fluorometric titration with riboflavin-binding apoprotein after acid-enzymatic hydrolysis, ascorbic acid, vitamin E (tocopherols), niacin, organic and hydroxycinnamic acids - by HPLC, flavonols - spectrophotometrically, minerals and trace elements - by atomic absorption method. . A significant variation in the chemical composition indices depending on the varietal characteristics was found. Strawberry fruit accumulated 8.5-12.0% soluble solids, 5.9-8.7% sugars, 1.5-2.1% of fibre, 0.78-1.12% pectin substances, 0.89-1.45% organic acid; 100 g contain 33.5-48.2 mg vitamin C, 18.3-108.5 mg anthocyanins, 0.024-0.041 mg vitamin B1, 0.012-0.029 mg vitamin B2, 0.10-0.26 mg niacin, 0.26-0.60 mg TE vitamin E, 102-270 mg potassium, 0.15-0.95 mg iron; total antioxidant activity ranged within 180.8-350.0 mg/100 g. High level of anthocyan accumulation (over 80.0 mg/100 g) was observed in Privlekatelnaya, Lakomaya and Feyerverk strawberry varieties. Urozhaynaya CGL, Privlekatelnaya and Lakomaya varieties were characterized by dessert taste. According to the results of the studies Privlekatelnaya variety stood out because of the high complex accumulation of vitamin C, anthocyanins, and high total antioxidant activity. These varieties are recommended for both fresh use and for freezing, as well as for production of functional foods. . The obtained data are of interest for further inclusion in the Tables of the chemical composition of Russian food products.
当前水果和浆果作物的研究领域之一是对其营养价值和微量营养素组成的研究。草莓宝贵的化学成分决定了其广泛的用途,不仅可作为新鲜水果,还可作为食品工业的原料。当代草莓品种的育种要求以及产量、大果型、果实重要的口感和营养品质,都要求有更高含量的生物活性物质。本研究的目的是对草莓果实的营养成分、生物活性化合物、矿物质元素及其抗氧化活性进行综合评估。研究对象是在伊万·米丘林科学中心培育的5个草莓品种(Lakomaya、Prazdnichnaya、Privlekatelnaya、Urozhaynaya CGL、Feyerverk)的浆果以及9个国外品种(Vima Zanta、Dukat、Zefir、Kama、Maryshka、Red Gauntlet、Festivalnaya romashka、Honey、Elsanta),这些品种在俄罗斯黑土地区具有种植前景。可溶性固形物含量通过折射法测定,糖分通过贝特朗法测定,果胶通过容量法测定,花青素通过pH差值分光光度法测定。抗氧化剂的总含量通过安培法测定,并以槲皮素重新计算。维生素B1的测定采用荧光硫色素法,维生素B2通过酸酶水解后用核黄素结合脱辅基蛋白进行荧光滴定测定,抗坏血酸、维生素E(生育酚)、烟酸、有机和羟基肉桂酸通过高效液相色谱法测定,黄酮醇通过分光光度法测定,矿物质和微量元素通过原子吸收法测定。发现化学成分指标因品种特性存在显著差异。草莓果实积累了8.5 - 12.0%的可溶性固形物、5.9 - 8.7%的糖分、1.5 - 2.1%的纤维、0.78 - 1.12%的果胶物质、0.89 - 1.45%的有机酸;每100克含有33.5 - 48.2毫克维生素C、18.3 - 108.5毫克花青素、0.024 - 0.041毫克维生素B1、0.012 - 0.029毫克维生素B2、0.1 – 0.26毫克烟酸