用人单克隆抗体测定人骨髓瘤蛋白的亚类分布。

Subclass distribution of human myeloma proteins as determined with monoclonal antibodies.

作者信息

Aucouturier P, Preud'Homme J L

机构信息

Laboratory of Immunology and Immunopathology (CNRS USA 1172), Poitiers University Hospital, France.

出版信息

Immunol Lett. 1987 Oct;16(1):55-7. doi: 10.1016/0165-2478(87)90061-7.

DOI:10.1016/0165-2478(87)90061-7

PMID:3123377

Abstract

Subclasses of 176 IgG and 62 IgA myeloma proteins were determined by indirect ELISA with monoclonal antibodies, as well as by an immunoblotting technique (for monoclonal IgG) and by immunoelectrophoresis against the lectin jacalin (for IgA). The subclass distribution of monoclonal IgG did not reflect mean normal serum levels of the correspondent isotypes, with an over-representation of IgG1 and IgG4 and an under-representation of IgG2 and IgG3 in myeloma. Similarly, IgA2 myeloma were clearly under-represented.

摘要

采用单克隆抗体间接酶联免疫吸附测定法（ELISA）、免疫印迹技术（用于单克隆IgG）以及针对凝集素红豆蔻的免疫电泳法（用于IgA），对176种IgG和62种IgA骨髓瘤蛋白的亚类进行了测定。单克隆IgG的亚类分布并不反映相应同型的正常血清平均水平，骨髓瘤中IgG1和IgG4比例过高，而IgG2和IgG3比例过低。同样，IgA2骨髓瘤的比例明显过低。