酶联免疫吸附测定法对饮食抗原的IgG亚类抗体进行定量分析。

ELISA quantitation of IgG subclass antibodies to dietary antigens.

作者信息

Husby S, Jensenius J C, Svehag S E

出版信息

J Immunol Methods. 1985 Oct 10;82(2):321-31. doi: 10.1016/0022-1759(85)90364-3.

Abstract

The IgG subclasses of human antibodies against 2 dietary antigens, ovalbumin (OA) and beta-lactoglobulin (BLG), were studied by ELISA using monoclonal anti-human IgG subclass antibodies. Under the assay conditions used, the anti-IgG subclass antibodies were subclass specific. Quantitative estimates of the subclass antibodies were obtained by reference to a 'capture' assay using F(ab')2 anti-light chain antibody as ligand and IgG myelomas as standards. The validity of these estimates was supported by antibody quantitation using the Farr assay. In healthy adults with serum anti-OA or anti-BLG antibodies, anti-OA antibodies were found mainly in the IgG1 (9/11) and IgG4 (6/11) subclasses, whereas 5 sera showed high levels of IgG2 antibodies. In contrast, the IgG subclass distribution of anti-BLG antibodies was predominantly IgG4 (10/10).

摘要

采用单克隆抗人IgG亚类抗体，通过酶联免疫吸附测定（ELISA）研究了人类针对两种膳食抗原——卵清蛋白（OA）和β-乳球蛋白（BLG）的抗体的IgG亚类。在所采用的检测条件下，抗IgG亚类抗体具有亚类特异性。通过使用F(ab')2抗轻链抗体作为配体、IgG骨髓瘤作为标准品的“捕获”测定法，对亚类抗体进行了定量估计。使用Farr测定法进行抗体定量，支持了这些估计的有效性。在具有血清抗OA或抗BLG抗体的健康成年人中，抗OA抗体主要存在于IgG1（9/11）和IgG4（6/11）亚类中，而5份血清显示IgG2抗体水平较高。相比之下，抗BLG抗体的IgG亚类分布主要为IgG4（10/10）。