Nauciel C, Ronco E, Guenet J L
Laboratoire de Microbiologie, Faculté de Médecine de Paris-Ouest, Garches, France.
Infect Immun. 1988 Feb;56(2):310-3. doi: 10.1128/iai.56.2.310-313.1988.
Infection of mice with a temperature-sensitive mutant of Salmonella typhimurium C5TS allowed the survival of genetically susceptible mice. The ability to mount a delayed-type hypersensitivity (DTH) response to sheep erythrocytes during infection with C5TS was studied in various inbred mouse strains, recombinant inbred strains derived from C57BL/6 (susceptible) and A/J (resistant) mice, and C3H congenic mice. Suppression of the DTH response to sheep erythrocytes was found in mice that carried the Itys allele, the H-2b haplotype, or both. These genes are known to increase susceptibility to S. typhimurium infection. In contrast, no DTH response suppression was observed in mouse strains that carried other genes that increased susceptibility to S. typhimurium, e.g., DBA/2 and C3H/HeJ. Apart from a transient suppression in A/J mice, the DTH responses of resistant mice (A/J and CBA) were normal or increased. The DTH response to sheep erythrocytes could be restored in immunodepressed mice by increasing the immunizing dose, suggesting the possible role of activated macrophages in depression of the DTH response.
用鼠伤寒沙门氏菌C5TS的温度敏感突变体感染小鼠，可使遗传易感小鼠存活。在感染C5TS期间，研究了各种近交系小鼠、源自C57BL/6（易感）和A/J（抗性）小鼠的重组近交系以及C3H同源基因小鼠对绵羊红细胞产生迟发型超敏反应（DTH）的能力。在携带Itys等位基因、H-2b单倍型或两者的小鼠中，发现对绵羊红细胞的DTH反应受到抑制。已知这些基因会增加对鼠伤寒沙门氏菌感染的易感性。相比之下，在携带其他增加对鼠伤寒沙门氏菌易感性的基因（如DBA/2和C3H/HeJ）的小鼠品系中，未观察到DTH反应受到抑制。除了A/J小鼠出现短暂抑制外，抗性小鼠（A/J和CBA）的DTH反应正常或增强。通过增加免疫剂量，免疫抑制小鼠对绵羊红细胞的DTH反应可以恢复，这表明活化巨噬细胞可能在DTH反应的抑制中发挥作用。
