Rashad Nearmeen M, Sayed Sally E, Sherif Mohamed H, Sitohy Mahmoud Z
Internal Medicine Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Biochemistry Department, Faculty of Science, Zagazig University, Zagazig, Egypt.
Diabetes Metab Syndr. 2019 May-Jun;13(3):2230-2235. doi: 10.1016/j.dsx.2019.05.027. Epub 2019 May 25.
Obesity is a major contributor to preventable disease and death across the globe. Obesity is complex. Although its risk factors are myriad and compounding, there is an urgent need for a deeper understanding of the way risk factors interact with each other. Leptin is a peptide regulates food intake and body weight. However, the notion of leptin as an anti-obesity hormone was called into question because obesity is typically associated with high leptin levels and not leptin deficiency thus, we aimed to measure leptin levels in obese female in correlation to anthropometric measures and to evaluate the impact of weight loss on its level and metabolic parameters.
case-control study enrolled 40 control groups, 50 obese women. We measured anthropometric measures BMI, Waist/hip ratio (WHR). Fat mass index (FMI%) and free fat mass index (FFMI%) were assessed by dual energy X-Ray absorptiometry (DEXA) The serum levels of leptin were measured by ELISA.
Our results revealed that serum leptin levels were higher in obese women compared to controls. Moreover, it was positively correlated to anthropometric measures, glycemic and lipid profile. Linear regression analysis revealed that BMI was the main independent studied parameters associated with serum leptin level among other clinical and laboratory biomarkers. Interestingly, after 12 weeks of following the Mediterranean diet (MD)-based weight loss program, serum leptin levels were decreased. Logistic regression analysis was performed to detect the main predictors' biomarkers associated with weight loss among obese women. We found that serum leptin and FMI% were an independent predictor of response with odds ratios of 1.69 and 1.64 respectively (P < 0.001), Receiver operating characteristic analyses revealed that the AUC of serum leptin in discriminating obese women from lean ones was 0.893 (95% CI = 0.815-0.917) with sensitivity = 90%, specificity = 96%, and the cutoff values was 36.32 ng/ml.
Serum leptin could be a valuable diagnostic marker of obesity and its comorbidities. Moreover, significant weight loss leads to decrease serum leptin levels and improvement of glycemic and lipid profiles.
肥胖是全球可预防疾病和死亡的主要原因。肥胖情况复杂。尽管其风险因素众多且相互交织，但迫切需要更深入地了解这些风险因素之间的相互作用方式。瘦素是一种调节食物摄入和体重的肽。然而，瘦素作为一种抗肥胖激素的概念受到了质疑，因为肥胖通常与高瘦素水平相关，而非瘦素缺乏。因此，我们旨在测量肥胖女性的瘦素水平，并将其与人体测量指标相关联，同时评估体重减轻对其水平和代谢参数的影响。
病例对照研究纳入了40个对照组和50名肥胖女性。我们测量了人体测量指标，如体重指数（BMI）、腰臀比（WHR）。通过双能X线吸收法（DEXA）评估脂肪量指数（FMI%）和去脂体重指数（FFMI%）。采用酶联免疫吸附测定法（ELISA）测量血清瘦素水平。
我们的结果显示，肥胖女性的血清瘦素水平高于对照组。此外，它与人体测量指标、血糖和血脂水平呈正相关。线性回归分析表明，在其他临床和实验室生物标志物中，BMI是与血清瘦素水平相关的主要独立研究参数。有趣的是，在遵循基于地中海饮食（MD）的减肥计划12周后，血清瘦素水平下降。进行逻辑回归分析以检测肥胖女性体重减轻的主要预测生物标志物。我们发现血清瘦素和FMI%是反应的独立预测因子，比值比分别为1.69和1.64（P<0.001）。受试者工作特征分析显示，血清瘦素区分肥胖女性和瘦女性的曲线下面积（AUC）为0.893（95%可信区间=0.815 - 0.917），敏感性为90%，特异性为96%，临界值为36.32 ng/ml。
血清瘦素可能是肥胖及其合并症的有价值诊断标志物。此外，显著的体重减轻会导致血清瘦素水平降低以及血糖和血脂水平改善。
