Parthenais E, Haskill S
J Natl Cancer Inst. 1979 Jun;62(6):1569-74.
The mitogenic response to phytohemagglutinin (PHA) by spleen cells from C3H/HeJ mice bearing either autochthonous tumors or early-generation transplanted spontaneous mammary tumors was depressed in some, but not all, tumor-bearing animals. T-cell hyporesponsiveness in both groups was associated with the larger (more rapidly growing) tumor burdens. The growth patterns of the transplanted tumors and the associated PHA-induced responses were not affected by the initial tumor cell dose or by the number of in vivo passages of the tumor cell lines. Suppressor cell activity was detected in the hyporesponsive spleens of mice bearing transplanted tumors. Depletion of phagocytic macrophages, rayon wool-adherent cells, or theta-positive lymphocytes did not remove the suppressor cell activity. The mitogenic response of some but not all hyporesponsive spleens from autochthonous tumor bearers was restored after removal of phagocytic macrophages. The results demonstrated the heterogeneity of the factor(s) influencing non-specific T-cell reactivity in animals bearing spontaneous mammary tumors. Furthermore, our data suggest that nonspecific immunosuppression does not precede spontaneous tumor appearance but is probably a late result of rapid, extensive tumor growth.
携带自体肿瘤或初代移植自发乳腺肿瘤的C3H/HeJ小鼠的脾细胞对植物血凝素（PHA）的促有丝分裂反应，在部分（而非全部）荷瘤动物中受到抑制。两组中的T细胞低反应性均与更大（生长更快）的肿瘤负荷相关。移植肿瘤的生长模式以及相关的PHA诱导反应，不受初始肿瘤细胞剂量或肿瘤细胞系体内传代次数的影响。在携带移植肿瘤小鼠的低反应性脾脏中检测到了抑制细胞活性。吞噬性巨噬细胞、粘在人造纤维上的细胞或θ阳性淋巴细胞的清除，并未消除抑制细胞活性。在清除吞噬性巨噬细胞后，部分（而非全部）来自自体肿瘤荷瘤小鼠的低反应性脾脏的促有丝分裂反应得以恢复。结果表明，影响自发乳腺肿瘤荷瘤动物非特异性T细胞反应性的因子具有异质性。此外，我们的数据表明，非特异性免疫抑制并非在自发肿瘤出现之前就已发生，而可能是快速、广泛肿瘤生长的晚期结果。
