Pope B L, Whitney R B, Levy J G, Kilburn D G
J Immunol. 1976 May;116(5):1342-6.
Spleen cells from mice bearing methylcholanthrene-induced sarcomas or a mammary adenocarcinoma suppressed the mitogen responses of normal spleen and lymph node cells. Lymph node cells from tumor bearers had no suppressive effects. Centrifugation of spleen cells layered on Hypaque-Ficoll (specific gravity of 1.08) produced a dense fraction which pelleted and a light fraction which was retained at the Hypaque-Ficoll-medium interface. Suppressive activity was not found in either fraction of normal spleen cells. In tumor-bearer spleen cells suppressor activity was greatly enriched in the light fraction. Treatment of the suppressor fraction with anti-theta or anti-Ig serum and complement did not remove suppressor activity. However, the suppressor cells were removed by passage through nylon wool or by carbonyl iron treatment. Also, the population which adhered to plastic Petri dishes contained the suppressor cell activity.
携带甲基胆蒽诱导肉瘤或乳腺腺癌的小鼠脾脏细胞抑制了正常脾脏和淋巴结细胞的丝裂原反应。肿瘤携带者的淋巴结细胞没有抑制作用。将脾脏细胞铺在聚蔗糖-泛影葡胺（比重1.08）上进行离心，产生一个沉淀的致密部分和一个保留在聚蔗糖-泛影葡胺-培养基界面的轻部分。正常脾脏细胞的这两个部分均未发现抑制活性。在肿瘤携带者的脾脏细胞中，抑制活性在轻部分中大量富集。用抗θ或抗Ig血清及补体处理抑制部分并不能去除抑制活性。然而，通过尼龙毛柱或羰基铁处理可去除抑制细胞。此外，附着在塑料培养皿上的细胞群体含有抑制细胞活性。
