Department of Microbiology, Faculty of Veterinary Medicine, Ankara University, Şehit Ömer Halisdemir Bulv. Dışkapı, 06110, Ankara, Turkey.
Arch Microbiol. 2019 Nov;201(9):1277-1284. doi: 10.1007/s00203-019-01694-0. Epub 2019 Jun 25.
Poultry vaccine programs are important for control of Salmonella infections. Although there are vaccines for Salmonella Enteritidis, Salmonella Typhimurium and Salmonella Typhi, there are no vaccines for Salmonella Infantis which has an increased rate in the world. In this study, it was aimed to generate aroA gene deleted mutant bacteria for the constitution of S. Infantis vaccine prototype and the in vitro characterisation of this bacterium. S. Infantis auxotrophic mutant which has a block at any step of chorismate pathway has been constituted for the first time in the world and it was determined that this bacterium gets susceptibility against some antibiotics and antimicrobial substances. It was also observed that the adhesion and invasion rate of mutant strain tenfold decreased in comparison with the field strain in cell culture assay. It is understood from the in vitro evaluation of this mutant strain that it can be used as a vaccine candidate in further vaccine development studies.
家禽疫苗计划对于控制沙门氏菌感染非常重要。虽然有针对肠炎沙门氏菌、伤寒沙门氏菌和 Typhi 沙门氏菌的疫苗，但目前还没有针对肠炎沙门氏菌的疫苗，而肠炎沙门氏菌在世界范围内的发病率正在增加。在这项研究中，我们旨在构建肠炎沙门氏菌疫苗原型的 aroA 基因缺失突变菌，并对该细菌进行体外特性分析。我们首次在世界范围内构建了任何一步都在分支酸途径中受阻的肠炎沙门氏菌营养缺陷型突变菌，并确定该突变菌对一些抗生素和抗菌物质的敏感性增加。在细胞培养试验中，还观察到突变株的粘附和侵袭率比野毒株降低了十倍。从该突变株的体外评估中可以理解，它可以作为进一步疫苗开发研究的候选疫苗。
