利用CRISPR对痘苗病毒基因组进行编辑
Vaccinia Virus Genome Editing Using CRISPR.
作者信息
Di Gioia Carmela, Yuan Ming, Wang Yaohe
机构信息
Barts Cancer Institute, Queen Mary University, London, UK.
出版信息
Methods Mol Biol. 2019;2023:109-117. doi: 10.1007/978-1-4939-9593-6_6.
PMID:31240673
Abstract
CRISPR/Cas9, an RNA-guided targeted genome editing system, can make precise, targeted modifications to the genome in living cells. Here we describe how this method can be used to efficiently edit the vaccinia virus genome enabling the insertion of transgene(s) specifically into a targeted site.
摘要
CRISPR/Cas9是一种RNA引导的靶向基因组编辑系统，可对活细胞中的基因组进行精确的靶向修饰。在此，我们描述了如何使用该方法有效编辑痘苗病毒基因组，从而能够将转基因特异性插入到靶向位点。
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