辅酶M与7-巯基庚酰苏氨酸磷酸的异二硫键在甲烷杆菌中将二氧化碳还原为甲烷过程中的生理重要性。
Physiological importance of the heterodisulfide of coenzyme M and 7-mercaptoheptanoylthreonine phosphate in the reduction of carbon dioxide to methane in Methanobacterium.
作者信息
Bobik T A, Wolfe R S
机构信息
Department of Microbiology, University of Illinois, Urbana 61801.
出版信息
Proc Natl Acad Sci U S A. 1988 Jan;85(1):60-3. doi: 10.1073/pnas.85.1.60.
Abstract
The heterodisulfide of the two coenzymes 2-mercaptoethanesulfonic acid (coenzyme M, HS-CoM) and N-(7-mercaptoheptanoyl)threonine O3-phosphate (HS-HTP) increased the rate of CO2 reduction to methane by cell extracts 42-fold. The stimulation resulted from activation of the initial step of methanogenesis, the production of formylmethanofuran from methanofuran and CO2. These results establish a role for this heterodisulfide (CoM-S-S-HTP) in the reduction of CO2 to formylmethanofuran. Evidence indicates that CoM-S-S-HTP is the labile intermediate that accounts for the coupling of the reduction of 2-(methylthio)ethanesulfonic acid by the methylreductase to formylmethanofuran biosynthesis, the "RPG effect." The heterodisulfide was found to be labile in cell extracts due to enzyme-catalyzed reduction and possibly thioldisulfide exchange.
摘要
两种辅酶——2-巯基乙烷磺酸（辅酶M，HS-CoM）和N-（7-巯基庚酰基）苏氨酸O3-磷酸（HS-HTP）的异二硫键，使细胞提取物将二氧化碳还原为甲烷的速率提高了42倍。这种刺激源于产甲烷作用第一步的激活，即从呋喃甲醇和二氧化碳生成甲酰呋喃甲醇。这些结果确立了这种异二硫键（CoM-S-S-HTP）在将二氧化碳还原为甲酰呋喃甲醇过程中的作用。有证据表明，CoM-S-S-HTP是不稳定中间体，它解释了甲基还原酶将2-（甲硫基）乙烷磺酸还原与甲酰呋喃甲醇生物合成的偶联，即“RPG效应”。由于酶催化还原以及可能的硫醇-二硫键交换，发现这种异二硫键在细胞提取物中不稳定。
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