Spolarics Z, Mucha I, Mandl J, Machovich R, Bánhegyi G, Antoni F, Garzó T
1st Institute of Biochemistry, Semmelweis University Medical School, Budapest, Hungary.
Prostaglandins Leukot Med. 1987 Oct;29(2-3):113-27. doi: 10.1016/0262-1746(87)90001-1.
Prostanoid synthesis was investigated in suspensions of isolated mouse hepatocytes and nonparenchymal liver cells. A stable metabolite of thromboxane A2 (TXB2) of prostacyclin (6-keto PGF1 alpha) and one of the prostaglandins (PGF2 alpha) was detected by radio-immuno-assay (RIA). Hepatocytes synthesized mainly TXB2, while smaller amounts of 6-keto PGF1 alpha and PGF2 alpha were detected during 60 min incubation. Homogenization of hepatocytes caused a slight increase of TXB2 production and provoked the synthesis of PGF2 alpha and 6-keto PGF1 alpha. The addition of arachidonate to hepatocytes did not influence prostanoid production at concentrations below 10-5M. Higher concentrations further increased TXB2 production and also increased the synthesis of 6-keto PGF1 alpha and PGF2 alpha. Nonparenchymal cells synthesized all the three types of prostanoids and homogenization of these cells did not result in a marked change. The addition of 10(-7)-10(-5)M arachidonate increased the TXB2, 6-keto PGF1 alpha and PGF2 alpha synthesis in nonparenchymal cells. No further increase was found at higher concentrations.
在分离的小鼠肝细胞和肝非实质细胞悬液中研究了前列腺素的合成。通过放射免疫测定法（RIA）检测到血栓素A2（TXB2）、前列环素（6-酮-前列腺素F1α）和一种前列腺素（前列腺素F2α）的稳定代谢产物。肝细胞主要合成TXB2，在60分钟孵育期间检测到少量的6-酮-前列腺素F1α和前列腺素F2α。肝细胞匀浆导致TXB2生成略有增加，并引发前列腺素F2α和6-酮-前列腺素F1α的合成。在浓度低于10^-5M时，向肝细胞中添加花生四烯酸不会影响前列腺素的生成。较高浓度进一步增加TXB2的生成，也增加6-酮-前列腺素F1α和前列腺素F2α的合成。肝非实质细胞合成所有三种类型的前列腺素，这些细胞的匀浆不会导致明显变化。添加10^-7 - 10^-5M花生四烯酸可增加肝非实质细胞中TXB2、6-酮-前列腺素F1α和前列腺素F2α的合成。在更高浓度下未发现进一步增加。
