Spolarics Z, Tanács B, Garzó T, Mandl J, Mucha I, Antoni F, Machovich R, Horváth I
Prostaglandins Leukot Med. 1984 Dec;16(3):379-88. doi: 10.1016/0262-1746(84)90194-x.
Prostanoid synthesis from 3H-arachidonic acid was compared in isolated parenchymal and nonparenchymal murine liver cells. The cells incorporated arachidonic acid into phospholipids but no prostanoid synthesis could be measured during 30 min incubation. Conditions necessary for prostanoid synthesis were different in parenchymal and nonparenchymal cells and the products were also different. Prostanoid synthesis could be induced by in vitro partial hepatectomy: parenchymal cells synthesized thromboxane A2 whereas nonparenchymal cells produced prostaglandin E2 and F2 alpha. Prostaglandin E2 and F2 alpha synthesis could be provoked also by homogenization of the nonparenchymal cells prepared from normal liver, while the homogenates of parenchymal cells prepared from normal liver did not synthesize thromboxane. Imidazole and indomethacin inhibited the production of thromboxane and prostaglandins, respectively. Our results suggest that the various cell types of the liver respond by the synthesis of different and specific prostanoids after the same injury.
在分离的小鼠肝实质细胞和非实质细胞中比较了从3H-花生四烯酸合成前列腺素的情况。这些细胞将花生四烯酸掺入磷脂中，但在30分钟的孵育过程中未检测到前列腺素的合成。前列腺素合成所需的条件在实质细胞和非实质细胞中不同，产物也不同。体外部分肝切除可诱导前列腺素合成：实质细胞合成血栓素A2，而非实质细胞产生前列腺素E2和F2α。正常肝脏制备的非实质细胞匀浆也可引发前列腺素E2和F2α的合成，而正常肝脏制备的实质细胞匀浆不合成血栓素。咪唑和吲哚美辛分别抑制血栓素和前列腺素的产生。我们的结果表明，肝脏的各种细胞类型在相同损伤后通过合成不同的特异性前列腺素作出反应。
