Liu Yuxin, Zhou Pengcheng, Da Honghong, Jia Huiyi, Bai Feifei, Hu Guodong, Zhang Baoxin, Fang Jianguo
State Key Laboratory of Applied Organic Chemistry &, College of Chemistry and Chemical Engineering, Lanzhou University, No. 222 South Tianshui Road, Lanzhou, 730000, China.
College of Chemistry & Materials Science, South-Central University for Nationalities, No. 708 Minyuan Road, Wuhan, 430074, China.
Chemistry. 2019 Aug 27;25(48):11228-11232. doi: 10.1002/chem.201901828. Epub 2019 Aug 1.
Herein, a strategy for the selective derivatization of 3-nitrotyrosine-containing proteins using the classic azo coupling reaction as the key step is described. This novel approach featured multiple advantages and was successfully applied to detect picomole levels of protein tyrosine nitration in biological samples.
本文描述了一种以经典偶氮偶联反应为关键步骤，对含3 - 硝基酪氨酸的蛋白质进行选择性衍生化的策略。这种新方法具有多种优势，并成功应用于检测生物样品中皮摩尔水平的蛋白质酪氨酸硝化。
