使用基于苯胺的氧化偶联策略直接检测含硝基酪氨酸的蛋白质。
Direct detection of nitrotyrosine-containing proteins using an aniline-based oxidative coupling strategy.
作者信息
Sangsuwan Rapeepat, Obermeyer Allie C, Tachachartvanich Phum, Palaniappan Krishnan K, Francis Matthew B
机构信息
Department of Chemistry, University of California, Berkeley, CA 94720-1460, USA.
出版信息
Chem Commun (Camb). 2016 Aug 21;52(65):10036-9. doi: 10.1039/c6cc04575h. Epub 2016 Jul 22.
Abstract
A convenient two-step method is described for the detection of nitrotyrosine-containing proteins. First, nitrotyrosines are reduced to aminophenols using sodium dithionite. Following this, an oxidative coupling reaction is used to attach anilines bearing fluorescence reporters or affinity probes. Features of this approach include fast reaction times, pmol-level sensitivity, and excellent chemoselectivity.
摘要
本文描述了一种用于检测含硝基酪氨酸蛋白质的便捷两步法。首先，使用连二亚硫酸钠将硝基酪氨酸还原为氨基酚。在此之后，利用氧化偶联反应连接带有荧光报告基团或亲和探针的苯胺。该方法的特点包括反应时间短、皮摩尔级灵敏度以及出色的化学选择性。
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