Hamid Q A, Rode J, Flanagan A M, Dhillon A P, Bishop A E, Stratton M, Evans D J, Polak J M
Department of Histochemistry, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
Virchows Arch A Pathol Anat Histopathol. 1988;412(3):267-72. doi: 10.1007/BF00737151.
Several types of metaplasia can occur in human bladder epithelium under certain pathological conditions. We investigated 65 cases of cystitis, associated with different types of metaplasia, for the presence of endocrine cells, using histochemical and immunocytochemical methods. Tissues were obtained at cystoscopy and were routinely fixed in 10% buffered formalin. Endocrine cells were demonstrated, between the epithelial cells, in 40 out of 50 cases of cystitis glandularis or cystica. These cells were positive by the Grimelius' silver impregnation technique and were immunoreactive for protein gene product (PGP 9.5), a new general neuroendocrine marker, chromogranin and serotonin. No endocrine cells were detected in any of the specimens of normal epithelium nor those showing squamous metaplasia. Eighteen of these cases showed prominent nerve bundles in the subepithelial tissue, as revealed by PGP immunoreactivity.
在某些病理条件下，人类膀胱上皮可发生几种类型的化生。我们采用组织化学和免疫细胞化学方法，对65例伴有不同类型化生的膀胱炎病例进行了内分泌细胞检测。组织在膀胱镜检查时获取，并常规固定于10%缓冲福尔马林中。在50例腺性膀胱炎或囊性膀胱炎病例中，有40例在上皮细胞之间发现了内分泌细胞。这些细胞经格里梅利乌斯银浸染技术呈阳性，对一种新的通用神经内分泌标志物蛋白基因产物（PGP 9.5）、嗜铬粒蛋白和5-羟色胺具有免疫反应性。在正常上皮标本或显示鳞状化生的标本中均未检测到内分泌细胞。其中18例病例显示上皮下组织中有明显的神经束，PGP免疫反应性证实了这一点。
