Korsholm E, Søgaard H
Department of Hygiene and Microbiology, Royal Veterinary and Agricultural University, Frederiksberg C, Denmark.
Zentralbl Bakteriol Mikrobiol Hyg B Umwelthyg Krankenhaushyg Arbeitshyg Prav Med. 1987 Oct;185(1-2):121-30.
Plate counts were compared with endotoxin (LPS) concentrations in 229 samples of unchlorinated drinking water. Plate counts were obtained on two different media (Kings agar B and R2A), and different fractions of LPS (total, free, and bound) were determined using a chromogenic LAL test kit. The closest correlation was found when total LPS was compared with R2A plate counts (r = 0.60), but based on regression analysis this relation was shown to be insufficient for practical use. Possible explanations for this discrepancy between plate counts and LPS are discussed, and it is concluded that the use of LPS as a surrogate indicator in drinking water bacteriology should not be rejected solely on the basis of a poor correlation with plate counts.
对229份未加氯的饮用水样本中的平板计数与内毒素（脂多糖，LPS）浓度进行了比较。在两种不同的培养基（金氏B琼脂和R2A）上进行平板计数，并使用显色鲎试剂检测试剂盒测定LPS的不同组分（总LPS、游离LPS和结合LPS）。当将总LPS与R2A平板计数进行比较时，发现相关性最强（r = 0.60），但基于回归分析，该关系在实际应用中被证明是不充分的。讨论了平板计数与LPS之间这种差异的可能原因，并得出结论，不应仅基于与平板计数的相关性差而拒绝将LPS用作饮用水细菌学中的替代指标。
