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饮用水细菌学中的菌落计数——培养基和方法的重要性

Colony counts in drinking water bacteriology--importance of media and methods.

作者信息

Korsholm E, Søgaard H

机构信息

Department of Hygiene and Microbiology, Royal Veterinary and Agricultural University, Frederiksberg C, Denmark.

出版信息

Zentralbl Bakteriol Mikrobiol Hyg B Umwelthyg Krankenhaushyg Arbeitshyg Prav Med. 1987 Oct;185(1-2):112-20.

PMID:3124373
Abstract

The productivity of three different plate count media were compared on unchlorinated drinking water samples. The R2A medium gave constantly higher counts than both Kings agar B and PCA, when incubated at 21 degrees C for 3 to 14 days. Spread plates were superior to pour plates for both R2A and Kings agar B, but for the R2A medium this difference diminished with time of incubation. It is suggested that this could partly be assigned to its content of sodium pyruvate as a H2O2 scavenger. Experiments with different strengths of the R2A medium showed that not only its complexity but also the low concentration of nutrients supplied is of significant importance to the productivity of the original formulation of this medium.

摘要

在未氯化的饮用水样本上比较了三种不同平板计数培养基的培养效果。当在21摄氏度下培养3至14天时,R2A培养基的计数始终高于King氏琼脂B和PCA。对于R2A和King氏琼脂B,涂布平板法优于倾注平板法,但对于R2A培养基,这种差异会随着培养时间的延长而减小。据推测,这可能部分归因于其作为过氧化氢清除剂的丙酮酸钠含量。用不同强度的R2A培养基进行的实验表明,不仅其复杂性,而且所提供的低营养浓度对该培养基原始配方的培养效果都具有重要意义。

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