Kulig B M
Medical Biological Laboratory TNO, Rijswijk, The Netherlands.
Zentralbl Bakteriol Mikrobiol Hyg B Umwelthyg Krankenhaushyg Arbeitshyg Prav Med. 1987 Oct;185(1-2):28-31.
The effects of CNS-active drugs and neurotoxic agents are often expressed as changes in coordinated movement. In order to quantitate such changes during prolonged chemical exposures, an automated technique for the routine measurement of coordinated hindlimb movement in the rat was developed. In this test, a color tv/microprocessor-based system was used to detect and analyze the movement of one of the rat's hindpaws as the rat placed its paw from one rung to another while walking in a rotating, motor-driven wheel. Test length was 90 sec and a wheel speed of 8.2 cm/sec was employed. Results from a 12 week study involving the ip administration of acrylamide (0, 3.6, 7.2 and 14.4 mg/kg) indicated that acrylamide produced a progressive decline in coordinated hindlimb movement beginning in Wk 6 of exposure. Although some recovery was evident following the termination of exposure, the performance of the highest dose group was still inferior to controls at the end of the 6 week recovery period. These results indicate that a video-based system for the measurement of coordinated movement can provide a rapid and reliable method for the study of prolonged neurotoxic exposures.
中枢神经系统活性药物和神经毒性剂的作用通常表现为协调运动的变化。为了在长期化学暴露期间量化此类变化，开发了一种用于常规测量大鼠后肢协调运动的自动化技术。在该测试中，使用基于彩色电视/微处理器的系统来检测和分析大鼠的一只后爪的运动，即当大鼠在由电机驱动的旋转轮中行走时将爪子从一个横档移到另一个横档的过程。测试时长为90秒，采用8.2厘米/秒的轮速。一项为期12周的研究结果，该研究涉及腹腔注射丙烯酰胺（0、3.6、7.2和14.4毫克/千克），表明丙烯酰胺从暴露第6周开始使后肢协调运动逐渐下降。尽管在暴露终止后有明显的恢复，但在6周恢复期结束时，最高剂量组的表现仍低于对照组。这些结果表明，基于视频的协调运动测量系统可为长期神经毒性暴露的研究提供一种快速且可靠的方法。
