Kamenova Ivanka, Borisova Anelija
Agrobioinstitute, 1164 Sofia, Bulgaria.
Institute of Agriculture, 2500 Kyustendil, Bulgaria.
Plant Pathol J. 2019 Jun;35(3):243-256. doi: 10.5423/PPJ.OA.09.2018.0189. Epub 2019 Jun 1.
Field surveys for (PPV) infection were conducted in stone fruit orchards all over Bulgaria. In total, 1168 out of 3020 leaf samples from cultivated spp. and wildly growing trees reacted positive for PPV in DASI-ELISA with the universal monoclonal antibody (MAb) 5B. Further ELISA analyses showed that 987 and 127 isolates belonged to PPV-M and PPV-D serotypes, respectively. The plum and showed 82.0% and 50.5% levels of infection, respectively followed by the peach (40.0%) and the apricot (32.0%). Five hundred fifty one PPV isolates were further typed by IC-RT-PCR with PPV-Rec, -M and -D-specific primers, targeting (Cter)NIb-(Nter) CP genome region, as 125 isolates were sequenced. The results revealed the presence of PPV-Rec, PPV-M and PPV-D and mixed infections of these strains. PPV-Rec was the most prevalent strain (49.0%), followed by PPV-M (40.1%), while PPV-D was the less spread strain (8.2%). PPV-Rec was the most common strain in plums, including the eight "old-aged" trees from the region of the first Sharka discovery. PPV-M was the most prevalent strain in peach and apricot. Phylogenetic analyses on (Cter)NIb-(Nter)CP of the isolates were performed. PPV-Rec isolates formed a homogeneous group, while PPV-M isolates split into PPV-Ma and PPV-Mb subgroups. Five separated clades were formed by the analyzed PPV-D isolates. Nucleotide sequences of the partial CP coding region of the analyzed isolates revealed a slightly higher intra-strain genetic variability in PPV-Rec and PPV-M isolates, while that of PPV-D strain isolates was higher from the reported for these strains.
在保加利亚各地的核果类果园开展了李痘病毒（PPV）感染情况的田间调查。总共从3020份栽培李属树种和野生李树的叶片样本中，有1168份在DASI - ELISA检测中与通用单克隆抗体（MAb）5B发生PPV阳性反应。进一步的ELISA分析表明，987份和127份分离株分别属于PPV - M和PPV - D血清型。李树和樱桃树的感染率分别为82.0%和50.5%，其次是桃树（40.0%）和杏树（32.0%）。使用针对（Cter）NIb -（Nter）CP基因组区域的PPV - Rec、 - M和 - D特异性引物，通过IC - RT - PCR对551份PPV分离株进行了进一步分型，其中125份分离株进行了测序。结果显示存在PPV - Rec、PPV - M和PPV - D以及这些菌株的混合感染。PPV - Rec是最普遍的菌株（49.0%），其次是PPV - M（40.1%），而PPV - D是传播较少的菌株（8.2%）。PPV - Rec是李树中最常见的菌株，包括来自首次发现沙卡病地区的8棵“老龄”树。PPV - M是桃树和杏树中最普遍的菌株。对分离株的（Cter）NIb -（Nter）CP进行了系统发育分析。PPV - Rec分离株形成一个同质组，而PPV - M分离株分为PPV - Ma和PPV - Mb亚组。分析的PPV - D分离株形成了5个不同的分支。分析分离株的部分CP编码区核苷酸序列显示，PPV - Rec和PPV - M分离株的菌株内遗传变异性略高，而PPV - D菌株分离株的遗传变异性高于这些菌株的报道值。
