Hemmati Chamran, Nikooei Mehrnoosh
Department of Agriculture, Minab Higher Education Center, University of Hormozgan, P.O.Box 3995, Bandar Abbas, Iran.
Plant Protection Research Group, University of Hormozgan, P.O.Box 3995, Bandar Abbas, Iran.
Plant Pathol J. 2019 Jun;35(3):274-279. doi: 10.5423/PPJ.NT.01.2019.0004. Epub 2019 Jun 1.
showing symptoms resembling those caused by phytoplasma were observed in Geno Mountain, Hormozgan Province, Iran, and were examined for phytoplasma presence by PCR assays. In addition, the essential oils hydrodistilled from the aerial parts of phytoplasma-infected and healthy plants have been analyzed and compared by GC and GC/MS. Phylogenetic and virtual RFLP analysis of the 16S rRNA gene sequences revealed that the phytoplasma associated with witches' broom (AsWB) was a strain of ' Phytoplasma aurantifolia'. The presence of the disease, however, induced a further enrichment (from 4.9 to 45.2%, a relative increase of 90%) of the entire monoterpene class as compared to the abundance in healthy samples. Conversely, a matching decrease in monoterpenoid (from 48.7 to 2%, a relative decrease of 90.2%) was observed in the infected plants. Besides the first report of phytoplasma infection of , the changes of its essential oils are reported.
在伊朗霍尔木兹甘省的杰诺山观察到出现了类似植原体引起的症状，并通过聚合酶链式反应（PCR）检测来检查是否存在植原体。此外，已对从感染植原体的植物和健康植物地上部分水蒸馏得到的精油进行了气相色谱（GC）和气相色谱-质谱联用（GC/MS）分析与比较。对16S rRNA基因序列进行的系统发育和虚拟限制性片段长度多态性（RFLP）分析表明，与扫帚病（AsWB）相关的植原体是“aurantifolia植原体”的一个菌株。然而，与健康样本中的丰度相比，该病的存在导致整个单萜类物质进一步富集（从4.9%增至45.2%，相对增加90%）。相反，在受感染植物中观察到单萜类化合物相应减少（从48.7%降至2%，相对减少90.2%）。除了首次报道 的植原体感染外，还报告了其精油的变化情况。
