Ogunye Ayokunle A, McNiece Ian K, Daniliuc Sharon, Genser-Nir Mira, Miropolski Yuliya-Yael, Fiorentini David, Kellner Joshua N
Department of Stem Cell Transplantation & Cellular Therapy, The University of Texas MD Anderson Cancer Center, Houston, TX, 77030 USA.
Biological Industries, Kibbutz Beit-Haemek, Israel.
Future Sci OA. 2019 Mar 22;5(5):FSO376. doi: 10.2144/fsoa-2018-0103. eCollection 2019 Jun.
Endothelial cells (ECs), isolated from peripheral blood (PB), bone marrow (BM) and cord blood (CB), are limited in numbers and expansion has had limited success. We used a novel serum-free medium (EndoGo) to evaluate effects on expansion of CB-derived ECs.
MATERIALS & METHODS: Flow cytometry and matrigel were used to determine expansion of ECs and for determination of the EC progenitor cell.
EndoGo™-containing cultures demonstrated superior expansion and stimulated proliferation of two distinct subpopulations, CD34CD31 and CD34CD31, which exhibited different morphology, phenotype and function. EndoGo also expanded the CB endothelial progenitor cells from freshly isolated CB.
These findings demonstrate the potential of EndoGo to expand CB ECs, which could generate increased numbers of ECs for therapeutic applications.
从外周血（PB）、骨髓（BM）和脐带血（CB）中分离出的内皮细胞（ECs）数量有限，且扩增效果有限。我们使用一种新型无血清培养基（EndoGo）来评估其对CB来源的ECs扩增的影响。
采用流式细胞术和基质胶来测定ECs的扩增情况以及EC祖细胞的测定。
含有EndoGo™的培养物显示出卓越的扩增能力，并刺激了两个不同亚群CD34CD31和CD34CD31的增殖，这两个亚群表现出不同的形态、表型和功能。EndoGo还能扩增新鲜分离的CB中的内皮祖细胞。
这些发现证明了EndoGo在扩增CB ECs方面的潜力，这可为治疗应用产生更多数量的ECs。
