Peng Chong, Shi Chaoshuo, Cao Xue, Li Yu, Liu Fufeng, Lu Fuping
Key Laboratory of Industrial Fermentation Microbiology, Education Ministry of China, Tianjin, China.
National Engineering Laboratory for Industrial Enzymes, Tianjin, China.
Front Bioeng Biotechnol. 2019 Jun 11;7:139. doi: 10.3389/fbioe.2019.00139. eCollection 2019.
Signal peptides are short peptides directing newly synthesized proteins toward the secretory pathway. These N-terminal signal sequences are ubiquitous to all prokaryotes and eukaryotes. Signal peptides play a significant role in recombinant protein production. Previous studies have demonstrated that the secretion amount of a given target protein varies significantly depending on the signal peptide that is fused to the protein. Signal peptide selection and signal peptide modification are the two main methods for the optimization of a recombinant protein secretion. However, the highly efficient signal peptide for a target protein with a specific bacterial expression host is not predictable so far. In this article, we collect several signal peptides that have previously performed well for recombinant protein secretion in gram-positive bacteria. We also discuss several factors influencing recombinant protein secretion efficiency in gram-positive bacteria. Signal peptides with a higher charge/length ratio in n-region, more consensus residues at the-3 and-1positions in c-region and a much higher proportion of coils are more likely to perform well in the secretion of recombinant proteins. These summaries can be utilized to the selection and directed modification of signal peptides for a given recombinant protein.
信号肽是引导新合成蛋白质进入分泌途径的短肽。这些N端信号序列在所有原核生物和真核生物中普遍存在。信号肽在重组蛋白生产中起着重要作用。先前的研究表明，给定目标蛋白的分泌量会因与该蛋白融合的信号肽的不同而有显著差异。信号肽选择和信号肽修饰是优化重组蛋白分泌的两种主要方法。然而，对于具有特定细菌表达宿主的目标蛋白而言，高效信号肽目前还无法预测。在本文中，我们收集了几种先前在革兰氏阳性菌中对重组蛋白分泌表现良好的信号肽。我们还讨论了影响革兰氏阳性菌中重组蛋白分泌效率的几个因素。在n区域具有较高电荷/长度比、在c区域的-3和-1位置具有更多共有残基以及具有更高比例卷曲结构的信号肽更有可能在重组蛋白分泌中表现良好。这些总结可用于给定重组蛋白信号肽的选择和定向修饰。
