Kleiner-Grote Gabriele R M, Risse Joe M, Friehs Karl
Fermentation Engineering Bielefeld University Bielefeld Germany.
Center for Biotechnology Bielefeld University Bielefeld Germany.
Eng Life Sci. 2018 Apr 14;18(8):532-550. doi: 10.1002/elsc.201700200. eCollection 2018 Aug.
The microorganism is commonly used for recombinant protein production. Despite several advantageous characteristics like fast growth and high protein yields, its inability to easily secrete recombinant proteins into the extracellular medium remains a drawback for industrial production processes. To overcome this limitation, a multitude of approaches to enhance the extracellular yield and the secretion efficiency of recombinant proteins have been developed in recent years. Here, a comprehensive overview of secretion mechanisms for recombinant proteins from is given and divided into three main sections. First, the structure of the cell envelope and the known natural secretion systems are described. Second, the use and optimization of different one- or two-step secretion systems for recombinant protein production, as well as further permeabilization methods are discussed. Finally, the often-overlooked role of cell lysis in secretion studies and its analysis are addressed. So far, effective approaches for increasing the extracellular protein concentration to more than 10 g/L and almost 100% secretion efficiency exist, however, the large range of optimization methods and their combinations suggests that the potential for secretory protein production from has not yet been fully realized.
这种微生物常用于重组蛋白生产。尽管它具有生长迅速和蛋白产量高等若干优势特性，但其难以将重组蛋白轻松分泌到细胞外培养基中的问题仍是工业生产过程中的一个缺陷。为克服这一限制，近年来已开发出多种提高重组蛋白细胞外产量和分泌效率的方法。在此，对来自[微生物名称未明确]的重组蛋白分泌机制进行全面概述，并分为三个主要部分。首先，描述[微生物名称未明确]的细胞膜结构和已知的天然分泌系统。其次，讨论用于重组蛋白生产的不同一步或两步分泌系统的使用和优化，以及进一步的通透化方法。最后，阐述细胞裂解在分泌研究中常被忽视的作用及其分析。到目前为止，存在将细胞外蛋白浓度提高到超过10 g/L且分泌效率几乎达到100%的有效方法，然而，大量的优化方法及其组合表明，来自[微生物名称未明确]的分泌蛋白生产潜力尚未得到充分实现。
