Department of Chemistry , Southern University of Science and Technology , Shenzhen , 518055 , China.
SUSTech Academy for Advanced Interdisciplinary Studies , Southern University of Science and Technology , Shenzhen , 518055 , China.
Anal Chem. 2019 Jul 2;91(13):8638-8643. doi: 10.1021/acs.analchem.9b02113. Epub 2019 Jun 19.
We report here an ionophore-based chemiluminescent platform for the selective detection of ions. This method functions on the basis of the ion-exchange between the target ion and the divalent organic cation lucigenin, which luminesces after reacting with hydrogen peroxide in alkaline conditions. Using the K ionophore valinomycin, chemiluminescent detection of K (from 10 nM to 1 M) was performed on both dichloromethane solutions and polymeric films. While the ionophores ensured excellent selectivity, the detection range could be adjusted with the ratio between the aqueous and the organic phases, and the divalent lucigenin ions also made the sensitivity for divalent target ions higher than conventional ionophore-based ion-selective optodes. The generalizability of the method was shown by changing the K ionophore into a Pb ionophore, which successfully realized the highly selective detection of Pb from 0.1 nM to 0.1 mM. Preliminary application of the method was demonstrated with the determination of K in diluted human blood serum (five samples), and the results agreed well with those obtained from potentiometric ion-selective electrodes.
我们在此报告了一种基于离子载体的化学发光平台，用于选择性检测离子。该方法基于目标离子与二价有机阳离子荧光素之间的离子交换，在碱性条件下与过氧化氢反应后发光。使用钾离子载体缬氨霉素，在二氯甲烷溶液和聚合物薄膜上均可对 K（从 10 nM 到 1 M）进行化学发光检测。虽然离子载体确保了出色的选择性，但检测范围可以通过水相和有机相的比例进行调节，并且二价荧光素离子也使对二价目标离子的灵敏度高于传统基于离子载体的离子选择性光极。通过将钾离子载体改为铅离子载体，该方法的通用性得到了证明，成功地实现了从 0.1 nM 到 0.1 mM 的高选择性检测铅。该方法的初步应用是通过测定稀释的人血清中的 K（五个样本）来证明的，结果与电位离子选择性电极的结果吻合良好。
