Hasan S M Kamrul, Li Ran, Wang Yichao, Reddy Narendra, Liu Wanshuang, Qiu Yiping, Jiang Qiuran
Key Laboratory of Textile Science &Technology, Ministry of Education, College of Textiles, Donghua University, Shanghai 201620, China.
Engineering Research Center of Technical Textiles, College of Textiles, Donghua University, Shanghai 201620, China.
Nanomaterials (Basel). 2019 Jun 26;9(7):918. doi: 10.3390/nano9070918.
The three-dimensional (3D) ultrafine fibrous scaffolds loaded with functional components can not only provide support to 3D tissue repair, but also deliver the components in-situ with small dosage and low fusion frequency. However, the conventional loading methods possess drawbacks such as low loading capacity or high burst release. In this research, an ultralow concentration phase separation (ULCPS) technique was developed to form 3D ultrafine gelatin fibers and, meanwhile, load an anti-inflammatory drug, diclofenac, with high capacities for the long-term delivery. The developed scaffolds could achieve a maximum drug loading capacity of 12 wt.% and a highest drug loading efficiency of 84% while maintaining their 3D ultrafine fibrous structure with high specific pore volumes from 227.9 to 237.19 cm/mg. The initial release at the first hour could be reduced from 34.7% to 42.2%, and a sustained linear release profile was observed with a rate of about 1% per day in the following 30 days. The diclofenac loaded in and released from the ULCPS scaffolds could keep its therapeutic molecular structure. The cell viability has not been affected by the release of drug when the loading was less than 12 wt.%. The results proved the possibility to develop various 3D ultrafine fibrous scaffolds, which can supply functional components in-situ with a long-term.
负载功能成分的三维（3D）超细纤维支架不仅可为3D组织修复提供支撑，还能以小剂量和低融合频率原位递送这些成分。然而，传统的负载方法存在诸如负载量低或突释率高的缺点。在本研究中，开发了一种超低浓度相分离（ULCPS）技术，以形成3D超细明胶纤维，同时高容量地负载一种抗炎药物双氯芬酸用于长期递送。所开发的支架在保持其具有227.9至237.19 cm/mg的高比孔体积的3D超细纤维结构的同时，可实现最大药物负载量为12 wt.%，最高药物负载效率为84%。最初1小时的释放率可从34.7%降低至42.2%，并且在接下来的30天内观察到持续的线性释放曲线，释放速率约为每天1%。负载于ULCPS支架并从中释放的双氯芬酸可保持其治疗分子结构。当负载量小于12 wt.%时，药物释放未影响细胞活力。结果证明了开发各种3D超细纤维支架的可能性，这些支架可长期原位提供功能成分。
