Yuan Huihua, Zhou Qihui, Li Biyun, Bao Min, Lou Xiangxin, Zhang Yanzhong
College of Chemistry, Chemical Engineering & Biotechnology, Donghua University, Shanghai 201620, People's Republic of China.
Biofabrication. 2015 Nov 5;7(4):045004. doi: 10.1088/1758-5090/7/4/045004.
Electrospinning has been widely used to produce ultrafine fibers in microscale and nanoscale; however, traditional electrospinning processes are currently beset by troublesome limitations in fabrication of 3D periodic porous structures because of the chaotic nature of the electrospinning jet. Here we report a novel strategy to print 3D poly(L-lactic acid) (PLLA) ultrafine fibrous scaffolds with the fiber diameter of approximately 2 μm by combining a stable jet electrospinning method and an X-Y stage technique. Our approach allows linearly deposited electrospun ultrafine fibers to assemble into 3D structures with tunable pore sizes and desired patterns. Process conditions (e.g., plotting speed, feeding rate, and collecting distance) were investigated in order to achieve stable jet printing of ultrafine PLLA fibers. The proposed 3D scaffold was successfully used for cell penetration and growth, demonstrating great potential for tissue engineering applications.
静电纺丝已被广泛用于制备微米级和纳米级的超细纤维；然而，由于静电纺丝射流的混沌特性，传统的静电纺丝工艺目前在制造三维周期性多孔结构时面临棘手的限制。在此，我们报告一种新颖的策略，通过结合稳定射流静电纺丝方法和X-Y平台技术，打印出纤维直径约为2μm的三维聚（L-乳酸）（PLLA）超细纤维支架。我们的方法允许线性沉积的静电纺超细纤维组装成具有可调孔径和所需图案的三维结构。为了实现PLLA超细纤维的稳定射流打印，研究了工艺条件（如绘图速度、进料速率和收集距离）。所提出的三维支架已成功用于细胞穿透和生长，显示出在组织工程应用中的巨大潜力。
