Instituto de Conservación y Mejora de la Agrodiversidad Valenciana, Universitat Politècnica de València, 46022 Valencia, Spain.
Int J Mol Sci. 2019 Jun 19;20(12):2990. doi: 10.3390/ijms20122990.
Eggplant varieties rich in bioactive chlorogenic acid along with less browning are preferred by consumers. Therefore, genetics of fruit phenolics, fruit flesh colour, and browning related traits were studied in the genotypes of eggplant, comprising of nine cultivated varieties and one accession of eggplant's primary genepool wild relative (INS2). These accessions were genotyped based on the 7335 polymorphic single-nucleotide polymorphisms (SNP) markers. After that, genotypes were crossed in half diallel fashion to produce 45 hybrids. The INS2 displayed the highest values for the total phenolics and chlorogenic acid content (CGA). For all of the biochemical traits studied, significant values of general and specific combining ability (GCA and SCA) effects were determined. The baker ratio estimates were high (>0.75) for all of the traits. Highly significant and positive heterosis (%) was determined for the dry matter, total phenolics, CGA, and area (%) of CGA content. The phenolics content of the fruit (total phenolics and CGA) was not significantly correlated with flesh colour and browning related traits. However, when the path coefficient analysis was performed considering the CGA as a dependent variable, it was determined that the flesh colour related traits most considerably affected the CGA. The genetic distance showed a diminutive correlation with the hybrid means, heterosis, and SCA values. Overall, this study provides important information regarding the underlying genetics of important biochemical traits of eggplant fruit.
消费者更喜欢富含生物活性绿原酸且不易褐变的茄子品种。因此，本研究对茄子的 10 个基因型（包括 9 个栽培品种和一个茄子初级基因库野生近缘种 INS2）进行了果实酚类物质、果肉颜色和褐变相关性状的遗传研究。这些基因型是基于 7335 个多态性单核苷酸多态性（SNP）标记进行分型的。之后，采用半双列杂交的方式对基因型进行杂交，产生了 45 个杂种。INS2 的总酚和绿原酸（CGA）含量最高。对于所有研究的生化性状，均确定了一般和特殊配合力（GCA 和 SCA）效应的显著值。所有性状的 baker 比值估计值都很高（>0.75）。干物质、总酚、CGA 和 CGA 含量的面积（%）均表现出显著的正杂种优势（%）。果实的酚类物质含量（总酚和 CGA）与果肉颜色和褐变相关性状没有显著相关性。然而，当考虑 CGA 为因变量进行通径系数分析时，发现与果肉颜色相关的性状对 CGA 的影响最大。遗传距离与杂种平均值、杂种优势和 SCA 值的相关性较小。总的来说，这项研究为茄子果实重要生化性状的遗传基础提供了重要信息。
