Fujian Provincial Key Laboratory of Crop Breeding by Design, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, China.
Theor Appl Genet. 2024 Sep 9;137(10):216. doi: 10.1007/s00122-024-04726-6.
A critical gene for leaf prickle development (LPD) in eggplant was mapped on chromosome E06 and was confirmed to be SmARF10B through RNA interference using a new genetic transformation technique called SACI developed in this study Prickles on eggplant pose challenges for agriculture and are undesirable in cultivated varieties. This study aimed to uncover the genetic mechanisms behind prickle formation in eggplant. Using the F and F populations derived from a cross between the prickly wild eggplant, YQ, and the prickle-free cultivated variety, YZQ, we identified a key genetic locus (LPD, leaf prickle development) on chromosome E06 associated with leaf prickle development through BSA-seq and QTL mapping. An auxin response factor gene, SmARF10B, was predicted as the candidate gene as it exhibited high expression in YQ's mature leaves, while being significantly low in YZQ. Downregulating SmARF10B in YQ through RNAi using a simple and efficient Agrobacterium-mediated genetic transformation method named Seedling Apical Cut Infection (SACI) developed in this study substantially reduced the size and density of leaf prickles, confirming the role of this gene in prickle development. Besides, an effective SNP was identified in SmARF10B, resulting in an amino acid change between YQ and YZQ. However, this SNP did not consistently correlate with prickle formation in eight other eggplant materials examined. This study sheds light on the pivotal role of SmARF10B in eggplant prickle development and introduces a new genetic transformation method for eggplant, paving the way for future research in this field.
茄子刺毛发育（LPD）的关键基因被定位在 E06 号染色体上，并通过本研究中新开发的称为 SACI 的遗传转化技术，利用 RNA 干扰确认其为 SmARF10B。茄子上的刺毛给农业带来了挑战，在栽培品种中是不受欢迎的。本研究旨在揭示茄子刺毛形成的遗传机制。利用来自刺毛野生茄子 YQ 和无刺毛栽培品种 YZQ 杂交的 F 和 F 群体，我们通过 BSA-seq 和 QTL 作图，在 E06 号染色体上鉴定出一个与叶片刺毛发育相关的关键遗传位点（LPD，叶片刺毛发育）。一个生长素响应因子基因 SmARF10B 被预测为候选基因，因为它在 YQ 的成熟叶片中表达量较高，而在 YZQ 中表达量显著较低。通过本研究中新开发的一种简单高效的农杆菌介导遗传转化方法，即 Seedling Apical Cut Infection（SACI），利用 RNAi 下调 YQ 中的 SmARF10B，显著减少了叶片刺毛的大小和密度，证实了该基因在刺毛发育中的作用。此外，在 SmARF10B 中还鉴定到一个有效的 SNP，导致 YQ 和 YZQ 之间氨基酸发生变化。然而，该 SNP 与在其他 8 种茄子材料中观察到的刺毛形成并不始终相关。本研究揭示了 SmARF10B 在茄子刺毛发育中的关键作用，并为茄子引入了一种新的遗传转化方法，为该领域的未来研究铺平了道路。
