Shandong Peanut Research Institute, Qingdao, Shandong Province 266000, China.
KeGene Science & Technology Co. Ltd., Tai'an, Shandong Province 271000, China.
Genes (Basel). 2019 Jul 15;10(7):536. doi: 10.3390/genes10070536.
Long non-coding RNAs (lncRNAs) are involved in various regulatory processes although they do not encode protein. Presently, there is little information regarding the identification of lncRNAs in peanut ( Linn.). In this study, 50,873 lncRNAs of peanut were identified from large-scale published RNA sequencing data that belonged to 124 samples involving 15 different tissues. The average lengths of lncRNA and mRNA were 4335 bp and 954 bp, respectively. Compared to the mRNAs, the lncRNAs were shorter, with fewer exons and lower expression levels. The 4713 co-expression lncRNAs (expressed in all samples) were used to construct co-expression networks by using the weighted correlation network analysis (WGCNA). LncRNAs correlating with the growth and development of different peanut tissues were obtained, and target genes for 386 hub lncRNAs of all lncRNAs co-expressions were predicted. Taken together, these findings can provide a comprehensive identification of lncRNAs in peanut.
长非编码 RNA（lncRNA）尽管不编码蛋白质，但参与了各种调节过程。目前，关于花生（ Linn.）lncRNA 的鉴定信息很少。在这项研究中，从属于 15 种不同组织的 124 个样本的大规模发表的 RNA 测序数据中鉴定了 50873 个花生 lncRNA。lncRNA 和 mRNA 的平均长度分别为 4335bp 和 954bp。与 mRNAs 相比，lncRNAs 更短，外显子更少，表达水平更低。使用加权相关网络分析（WGCNA），使用 4713 个共表达 lncRNA（在所有样本中表达）构建共表达网络。获得了与不同花生组织生长发育相关的 lncRNA，并预测了所有 lncRNA 共表达的 386 个枢纽 lncRNA 的靶基因。总之，这些发现可以为花生中的 lncRNA 提供全面的鉴定。
