Bast A, Haenen G R, Savenije-Chapel E M
Department of Pharmacochemistry, Free University, Subfaculty of Chemistry, Amsterdam, The Netherlands.
Arzneimittelforschung. 1987 Sep;37(9):1043-5.
Glutathione (GSH, 1 mmol/l) inhibits Fe2+/ascorbic acid induced liver microsomal lipid peroxidation. Oxidized GSH (GSSG, 1 mmol/l) did not affect rate or extent of lipid peroxidation. The inhibition by GSH seems specific since the sulfhydryl agent sodium 2-mercaptoethanesulfonate (mesna) gave a slight stimulation of lipid peroxidation. This stimulation is probably explained by a reduction by mesna of dehydroascorbic acid which is formed during the incubation, into ascorbic acid. Remarkably, mesna (1 mmol/l) added together with GSSG (1 mmol/l) produced the same inhibition as with 1 mmol/l GSH alone. This can be explained by direct reduction of GSSG to GSH by mesna. This is corroborated in experiments in which GSH is measured directly. Dimesna did not show an effect on lipid peroxidation. In the protective action of mesna against reactive substances its ability to reduce GSSG should be appreciated.
谷胱甘肽（GSH，1毫摩尔/升）可抑制Fe2+/抗坏血酸诱导的肝微粒体脂质过氧化。氧化型谷胱甘肽（GSSG，1毫摩尔/升）对脂质过氧化的速率或程度没有影响。GSH的抑制作用似乎具有特异性，因为巯基试剂2-巯基乙烷磺酸钠（美司钠）对脂质过氧化有轻微的促进作用。这种促进作用可能是由于美司钠将孵育过程中形成的脱氢抗坏血酸还原为抗坏血酸所致。值得注意的是，1毫摩尔/升美司钠与1毫摩尔/升GSSG一起添加时产生的抑制作用与单独使用1毫摩尔/升GSH时相同。这可以通过美司钠将GSSG直接还原为GSH来解释。在直接测量GSH的实验中得到了证实。二巯基丁二酸钠对脂质过氧化没有影响。在美司钠对活性物质的保护作用中，其还原GSSG的能力应得到重视。
