急性给予3,3'-二氯联苯胺对大鼠肝脏酶促和非酶促微粒体脂质过氧化及抗氧化状态的影响。
Effect of acute 3,3'-dichlorobenzidine administration on rat hepatic enzymic and nonenzymic microsomal lipid peroxidation and antioxidant status.
作者信息
Iba M M
出版信息
Res Commun Chem Pathol Pharmacol. 1987 May;56(2):243-52.
The effect of pretreatment with 3,3'-dichlorobenzidine (DCB) on (i) enzymic and nonenzymic lipid peroxidation in hepatic microsomes--as measured by malondialdehyde (MDA) formation, and (ii) hepatic antioxidant status--as measured by the contents of vitamin E and reduced glutathione (GSH) and the activity of glutathione peroxidase, was examined in the rat. DCB-pretreatment (20 mg/kg/day, ip, for 2 days) caused an increase in NADPH-dependent (enzymic) lipid peroxidation, but had no effect on the linoleic acid hydroperoxide-dependent (nonenzymic) lipid peroxidation in microsomes. DCB-pretreatment also caused a 44% decrease in the content of vitamin E in microsomes, but had no effect on the content of GSH or the activities of glutathione peroxidases in the liver. The DCB-induced increase in in vitro microsomal lipid peroxidation is interpreted as resulting from the diminution of vitamin E induced by DCB in vivo.
研究了用3,3'-二氯联苯胺（DCB）预处理对大鼠（i）肝微粒体中酶促和非酶促脂质过氧化作用（通过丙二醛（MDA）形成来衡量）以及（ii）肝脏抗氧化状态（通过维生素E、还原型谷胱甘肽（GSH）含量和谷胱甘肽过氧化物酶活性来衡量）的影响。DCB预处理（20毫克/千克/天，腹腔注射，持续2天）导致依赖NADPH的（酶促）脂质过氧化增加，但对微粒体中依赖亚油酸氢过氧化物的（非酶促）脂质过氧化没有影响。DCB预处理还导致微粒体中维生素E含量降低44%，但对肝脏中GSH含量或谷胱甘肽过氧化物酶活性没有影响。DCB诱导的体外微粒体脂质过氧化增加被解释为是由于DCB在体内导致维生素E减少所致。
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