Department of Chemical Engineering, University of Huddersfield, UK.
Department of Biological and Geographical Sciences, University of Huddersfield, UK.
J Sci Food Agric. 2019 Nov;99(14):6191-6198. doi: 10.1002/jsfa.9891. Epub 2019 Jul 27.
Pectin characteristics from different parts of lemon fruit (Citrus limon L.) were studied as a basis for assessing their suitability for functional applications. Pectin was extracted from lemon albedo, lemon core parts and membranes, and lemon extract using an aqueous extraction protocol. The composition and structural properties of the isolated pectins were examined by means of complementary analytical methods to assess their molecular characteristics for potential industrial applications.
The isolation protocol yielded pectins that were predominantly composed of galacturonic acid, with differences in the degree of methylation and neutral sugars content, and with low protein content, indicating high-purity materials. The same extraction protocol resulted in differences in yield and purity between the three different parts of lemon fruit, and in structural variations in the pectin backbone, as evidenced by differences in sugar composition and molecular weight. Solutions of the isolated lemon pectins exhibited pseudoplastic behavior. Macromolecular characterization showed that the lemon extract pectin had the highest molecular weight and hydrodynamic volume, followed by lemon core and lemon albedo pectin.
The work demonstrates that pectins with distinct structural properties may be extracted from different parts of lemon wastes and used for different technological purposes. © 2019 Society of Chemical Industry.
为评估其在功能应用方面的适用性,对柠檬(Citrus limon L.)不同部位的果胶特性进行了研究。采用水提方法从柠檬白皮、柠檬芯和柠檬膜以及柠檬提取物中提取果胶。采用互补分析方法对分离得到的果胶的组成和结构特性进行了检测,以评估其在潜在工业应用方面的分子特性。
该分离方案得到的果胶主要由半乳糖醛酸组成,在甲基化程度和中性糖含量方面存在差异,且蛋白质含量低,表明是高纯度的材料。相同的提取方案导致柠檬果实的三个不同部位之间在产率和纯度上存在差异,并导致果胶主链的结构发生变化,表现在糖组成和分子量方面的差异。分离得到的柠檬果胶溶液表现出假塑性行为。大分子特性分析表明,柠檬提取物果胶的分子量和流体力学体积最高,其次是柠檬芯和柠檬白皮果胶。
该研究表明,可以从柠檬废料的不同部位提取具有不同结构特性的果胶,并用于不同的技术目的。 © 2019 英国化学学会。