Anees Muhammad, Abid Muhammad, Chohan Sobia, Jamil Muhammad, Ahmed Nadeem, Zhang Lixin, Rha Eui Shik
Department of Microbiology, Kohat University of Science & Technology , Kohat , Pakistan.
Department of Plant Pathology, Baha ud Din Zakaria University , Multan , Pakistan.
Pol J Microbiol. 2019;68(2):211-216. doi: 10.33073/pjm-2019-021.
is a soil-borne fungus causing a wide range of plants diseases. strain T30 has previously been reported as antagonistic against . Although there are a few studies about the influence of strains on the density in a pathosystem in the presence of plant hosts, this report for the first time comprehensively describes effects of a strain on the population density of in the soil microcosmic conditions. The population dynamics of were followed in the autoclaved and non-autoclaved soils in artificially prepared microcosms up to day 25 after co-inoculation with in the variable ratios (R1/T1; R1/T0.1; R1/T0.01 of /). The population density of was evaluated by qPCR. In the autoclaved soil, target DNA copies of increased in the control samples from 1 × 10 to 6.5 × 10. At R1/T0.01, the number of target DNA copies were not significantly changed until day 11; however, it decreased by around five times at day 25. At R1/T0.1 and R1/T1, the number of DNA copies was reduced to 2.1 × 10 and 7.6 × 10 at day 11, respectively and the reduction was as much as 17 times at day 25. In the non-autoclaved soil, the number of the fungal cells decreased at day 25 whether inoculated or not with indicating a general suppression by the soil microbiome. In brief, significantly inhibited the growth of in the soil and there was a general suppressive effect of the natural microbiome. is a soil-borne fungus causing a wide range of plants diseases. strain T30 has previously been reported as antagonistic against . Although there are a few studies about the influence of strains on the density in a pathosystem in the presence of plant hosts, this report for the first time comprehensively describes effects of a strain on the population density of in the soil microcosmic conditions. The population dynamics of were followed in the autoclaved and non-autoclaved soils in artificially prepared microcosms up to day 25 after co-inoculation with in the variable ratios (R1/T1; R1/T0.1; R1/T0.01 of /). The population density of was evaluated by qPCR. In the autoclaved soil, target DNA copies of increased in the control samples from 1 × 10 to 6.5 × 10. At R1/T0.01, the number of target DNA copies were not significantly changed until day 11; however, it decreased by around five times at day 25. At R1/T0.1 and R1/T1, the number of DNA copies was reduced to 2.1 × 10 and 7.6 × 10 at day 11, respectively and the reduction was as much as 17 times at day 25. In the non-autoclaved soil, the number of the fungal cells decreased at day 25 whether inoculated or not with indicating a general suppression by the soil microbiome. In brief, significantly inhibited the growth of in the soil and there was a general suppressive effect of the natural microbiome.
是一种土传真菌，可引发多种植物病害。T30菌株此前被报道具有抗……的特性。尽管有一些关于……菌株在存在植物宿主的病理系统中对……密度影响的研究，但本报告首次全面描述了一种……菌株在土壤微观条件下对……种群密度的影响。在人工制备的微观环境中的灭菌土壤和未灭菌土壤中，跟踪了……与……以不同比例（……的R1/T1；R1/T0.1；R1/T0.01）共同接种后直至第25天的种群动态。通过qPCR评估……的种群密度。在灭菌土壤中，对照样品中……的目标DNA拷贝数从1×10增加到6.5×10。在R1/T0.01时，目标DNA拷贝数直到第11天没有显著变化；然而，在第25天减少了约五倍。在R1/T0.1和R1/T1时，DNA拷贝数在第11天分别降至2.1×10和7.6×10，在第25天减少多达17倍。在未灭菌土壤中，无论是否接种……，真菌细胞数量在第25天均减少，表明受到土壤微生物群落的普遍抑制。简而言之，……显著抑制了土壤中……的生长，并且天然微生物群落具有普遍的抑制作用。是一种土传真菌，可引发多种植物病害。T30菌株此前被报道具有抗……的特性。尽管有一些关于……菌株在存在植物宿主的病理系统中对……密度影响的研究，但本报告首次全面描述了一种……菌株在土壤微观条件下对……种群密度的影响。在人工制备的微观环境中的灭菌土壤和未灭菌土壤中，跟踪了……与……以不同比例（……的R1/T1；R1/T0.1；R1/T0.01）共同接种后直至第25天的种群动态。通过qPCR评估……的种群密度。在灭菌土壤中，对照样品中……的目标DNA拷贝数从1×10增加到6.5×10。在R1/T0.01时，目标DNA拷贝数直到第11天没有显著变化；然而，在第25天减少了约五倍。在R1/T0.1和R1/T1时，DNA拷贝数在第11天分别降至2.1×10和7.6×10，在第25天减少多达17倍。在未灭菌土壤中，无论是否接种……，真菌细胞数量在第25天均减少，表明受到土壤微生物群落的普遍抑制。简而言之，……显著抑制了土壤中……的生长，并且天然微生物群落具有普遍的抑制作用。
