College of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China.
College of Life Sciences, Chongqing Normal University, Chongqing 401331, China.
Int J Biol Macromol. 2019 Sep 1;136:1106-1111. doi: 10.1016/j.ijbiomac.2019.06.186. Epub 2019 Jun 25.
The sterilization process, due to its immense energy consumption, high facilities investment, and loss of raw materials by caramelization, during industrial production has drawn much attention. In this study, a methanol-resistant mutant strain, Chaetomium globosum ALE20, was obtained following 20 cycles of adaptive laboratory evolution process. The titer of anticancer polysaccharide (GCP-M) from C. globosum ALE20 reached 9.2 g/L with glycerol as sole carbon source using non-sterilized and fed-batch fermentation strategy. This titer represents a 200% increase compared with the 3.3 g/L attained with batch fermentation. The GCP-M monosaccharide was comprised of galactose, glucose, mannose and glucuronic acid, in a molar ratio of 3.83:66.37:3.26:1.95, respectively, and its weight-average molecular weight and polydispersity were 3.796 × 10 Da and 1.060, respectively. This work presents an ideal alternative and safer fermentation process without sterilization, and a useful approach for enhancing industrial production.
由于能源消耗巨大、设备投资高以及原料在焦糖化过程中的损失，灭菌过程在工业生产中引起了广泛关注。本研究通过 20 轮适应性实验室进化过程，获得了一株甲醇抗性突变株，即 Chaetomium globosum ALE20。以甘油为唯一碳源，采用非灭菌和分批补料发酵策略，从 C. globosum ALE20 中获得的抗癌多糖（GCP-M）的产量达到 9.2 g/L，比分批发酵的 3.3 g/L 提高了 200%。GCP-M 的单糖组成分别为半乳糖、葡萄糖、甘露糖和葡萄糖醛酸，摩尔比为 3.83:66.37:3.26:1.95，重均分子量和多分散性分别为 3.796×10 Da 和 1.060。这项工作提出了一种理想的替代和更安全的发酵工艺，无需灭菌，为提高工业生产提供了一种有用的方法。
