Li Shiwei, Wang Yingna, Dun Weipeng, Han Wanqing, Xu Chunping, Sun Qi, Wang Zichao
College of Life Sciences and Agronomy, Zhoukou Normal University, Zhoukou, China.
College of Food Science and Technology, Henan University of Technology, Zhengzhou, China.
Front Nutr. 2022 Jul 19;9:941524. doi: 10.3389/fnut.2022.941524. eCollection 2022.
Similar to the enzymatic process, there might also be an active fragment in polysaccharides, how to obtain is important for investigating the bioactivity and pharmacological mechanism of polysaccharides. Presently, a endophytic fungus CGMCC 6882 polysaccharide [Genistein Combined Polysaccharide (GCP)] was degraded by ultrasonic treatment, two polysaccharide fragments of GCP-F1 and GCP-F2 were obtained. Physicochemical results showed that GCP-F1 and GCP-F2 had the same monosaccharide composition of arabinose, galactose, glucose, xylose, mannose, and glucuronic acid as compared to GCP with slightly different molar ratios. However, weight-average molecular weights of GCP-F1 and GCP-F2 decreased from 8.093 10 Da (GCP) to 3.158 × 10 Da and 1.027 × 10 Da, respectively. scavenging assays illustrated that GCP-F1 and GCP-F2 had higher antioxidant activity against 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, superoxide anions, and hydroxyl radical than GCP, the order was GCP < GCP-F1 < GCP-F2. Meanwhile, antibacterial tests showed that ultrasonic degradation increased the antibacterial activity of GCP-F1 as compared to GCP, but GCP-F2 almost lost its antibacterial activity with further ultrasound treatment. Changes in the antioxidant and antibacterial activities of GCP-F1 and GCP-F2 might be related to the variation of their molecular weights.
与酶促过程类似，多糖中可能也存在活性片段，如何获得这些片段对于研究多糖的生物活性和药理机制至关重要。目前，通过超声处理降解了一种内生真菌CGMCC 6882多糖[染料木素复合多糖（GCP）]，获得了两个多糖片段GCP-F1和GCP-F2。物理化学结果表明，与GCP相比，GCP-F1和GCP-F2具有相同的阿拉伯糖、半乳糖、葡萄糖、木糖、甘露糖和葡萄糖醛酸单糖组成，只是摩尔比略有不同。然而，GCP-F1和GCP-F2的重均分子量分别从8.093×10⁴Da（GCP）降至3.158×10³Da和1.027×10³Da。清除自由基实验表明，GCP-F1和GCP-F2对2,2'-偶氮二（3-乙基苯并噻唑啉-6-磺酸）（ABTS）自由基、2,2-二苯基-1-苦基肼（DPPH）自由基、超氧阴离子和羟基自由基的抗氧化活性高于GCP，顺序为GCP＜GCP-F1＜GCP-F2。同时，抗菌测试表明，与GCP相比，超声降解提高了GCP-F1的抗菌活性，但进一步超声处理后，GCP-F2几乎失去了抗菌活性。GCP-F1和GCP-F2抗氧化和抗菌活性的变化可能与其分子量的变化有关。
