Department of Analytical Chemistry, School of Sciences, China Pharmaceutical University, Nanjing 210009, Jiangsu, China.
Department of Analytical Chemistry, School of Sciences, China Pharmaceutical University, Nanjing 210009, Jiangsu, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2019 Aug 15;1124:273-283. doi: 10.1016/j.jchromb.2019.06.007. Epub 2019 Jun 6.
In this work, quercetin (Que) molecular imprinted polymer (MIP) material decorated on magnetic graphene oxide (MGO) with high performance was prepared for the first time using a surface-imprinting technique. Magnetic graphene oxide was synthesized using the solvothermal route. Methacrylic acid (MAA) was used as functional monomer, ethylene glycol dimethyl acrylate (EGDMA) as cross-linker; Que. was used as template, for the decoration with MIP. The prepared nanocomposite was examined by different characterization methods including fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), vibrating sample magnetometer (VSM). The adsorption performance was investigated. MGO-MIP was found to have high loading (369 mg g) and selective capacity making the nanomaterial more performant than previous similar reported nanomaterials. The determination of Que. was carried out by mean of magnetic solid phase extraction method coupled with high-performance liquid chromatography (HPLC) and the extraction conditions studies were also performed out. Under the optimized conditions, MGO-MIP showed great performance for the extraction, separation and determination of Que. in green tea and serum samples, compared to the flavonoid analogs luteolin (Lut) and rutin (Rut) in the same matrix samples.
本工作首次采用表面印迹技术制备了具有高性能的槐属分子印迹聚合物（MIP）材料修饰的磁性氧化石墨烯（MGO）。磁性氧化石墨烯是通过溶剂热法合成的。选用甲基丙烯酸（MAA）作为功能单体，二乙二醇二甲基丙烯酸酯（EGDMA）作为交联剂；槐属作为模板，用于 MIP 的修饰。通过傅里叶变换红外光谱（FT-IR）、扫描电子显微镜（SEM）、振动样品磁强计（VSM）等不同的表征方法对制备的纳米复合材料进行了检查。考察了吸附性能。发现 MGO-MIP 具有高负载量（369mg/g）和选择性容量，使该纳米材料比以前报道的类似纳米材料性能更优。采用高效液相色谱（HPLC）结合磁性固相萃取法测定槐属。并对提取条件进行了研究。在优化条件下，与同一基质样品中的黄酮类似物木犀草素（Lut）和芦丁（Rut）相比，MGO-MIP 对绿茶和血清样品中槐属的提取、分离和测定具有优异的性能。
