镉通过诱导玉米根中的活性氧来激活 ZmMPK3-1 和 ZmMPK6-1。

Cadmium activates ZmMPK3-1 and ZmMPK6-1 via induction of reactive oxygen species in maize roots.

机构信息

Key Laboratory of Forest Resources Conservation and Utilization in the Southwest Mountains of China (Southwest Forestry University), Ministry of Education, College of Forestry, Southwest Forestry University, 300 Bailong Si, Kunming, 650224, Yunnan, People's Republic of China.

School of Ecology and Landscape Architecture, Dezhou University, 566 West University Road, Dezhou, 253023, Shandong, China.

出版信息

Biochem Biophys Res Commun. 2019 Aug 27;516(3):747-752. doi: 10.1016/j.bbrc.2019.06.116. Epub 2019 Jun 26.

DOI:10.1016/j.bbrc.2019.06.116

PMID:31253404

Abstract

Both mitogen-activated protein kinase (MAPK) cascades and reactive oxygen species (ROS) are critical to signaling in eukaryotes. Cadmium induces MAPK activation and ROS production in plants. This study aims to identify specific MAPKs activated by CdCl in maize roots, and examine the relationship between MAPK activation and ROS production under CdCl treatment. Using in-gel kinase assays, immunoprecipitation, and immunoblot analysis, we identified 43 and 45 kDa ERK-like MAPKs in response to CdCl. Further analysis revealed that ZmMPK3-1 and ZmMPK6-1 correspond to the 43 and 45 kDa MAPKs, respectively. In addition, CdCl induced ROS production prior to the activation of ZmMPK3-1 and ZmMPK6-1. Inhibition of ROS attenuated Cd-activation of ZmMPK3-1 and ZmMPK6-1, whereas inhibition of MAPK signaling did not disturb Cd-induced ROS production. Collectively, these results indicate that, in maize roots, cadmium stress activates ZmMPK3-1 and ZmMPK6-1 via ROS induction.

摘要

丝裂原活化蛋白激酶（MAPK）级联和活性氧（ROS）对于真核生物的信号转导至关重要。镉在植物中诱导 MAPK 的激活和 ROS 的产生。本研究旨在鉴定 CdCl 诱导的玉米根中特定的 MAPK，以及在 CdCl 处理下考察 MAPK 激活与 ROS 产生之间的关系。通过胶内激酶测定、免疫沉淀和免疫印迹分析，我们鉴定出 43 和 45 kDa ERK 样 MAPKs 对 CdCl 的响应。进一步的分析表明，ZmMPK3-1 和 ZmMPK6-1 分别对应于 43 和 45 kDa 的 MAPKs。此外，CdCl 在 ZmMPK3-1 和 ZmMPK6-1 的激活之前诱导 ROS 的产生。ROS 的抑制减弱了 Cd 对 ZmMPK3-1 和 ZmMPK6-1 的激活，而 MAPK 信号的抑制并没有干扰 Cd 诱导的 ROS 产生。总的来说，这些结果表明，在玉米根中，镉胁迫通过 ROS 的诱导激活 ZmMPK3-1 和 ZmMPK6-1。