Shu Shin La, Paruchuru Lakshmi Bhargavi, Tay Neil Quanwei, Chua Yen Leong, Foo Adeline Shen Yun, Yang Chris Maolin, Liong Ka Hang, Koh Esther Geok Liang, Lee Angeline, Nechushtan Hovav, Razin Ehud, Kemeny David Michael
Immunology Programme, Department of Microbiology, Centre for Life Sciences, National University of Singapore, #03-09, 28 Medical Drive, Singapore 117456, Singapore; NUS-HUJ-CREATE, 1 Create Way, Innovation Wing #03-09, Singapore 138602, Singapore.
Department of Biochemistry and Molecular Biology, Institute for Medical Research Israel-Canada, Hebrew University of Jerusalem, Jerusalem 91120, Israel.
iScience. 2019 Jun 28;16:524-534. doi: 10.1016/j.isci.2019.05.045. Epub 2019 Jun 4.
The significance of intracellular ApA levels over immune activity of dendritic cells (DCs) has been studied in Nudt2/CD11c-cre mice. The transgenic mice have been generated by crossing floxed NUDT2 gene mice with DC marker CD11c recombinase (cre) mice. The DCs derived from these mice have higher levels of ApA (≈30-fold) compared with those derived from Nudt2 mice. Interestingly, the elevated ApA in DCs has led them to possess higher motility and lower directional variability. In addition, the DCs are able to enhance immune protection indicated by the higher cross-presentation of antigen and priming of CD8 OT-I T cells. Overall, the study denotes prominent impact of ApA over the functionality of DCs. The Nudt2/CD11c-cre mice could serve as a useful tool to study the influence of ApA in the critical immune mechanisms of DCs.
在Nudt2/CD11c-cre小鼠中研究了细胞内ApA水平对树突状细胞（DCs）免疫活性的意义。通过将携带floxed NUDT2基因的小鼠与DC标志物CD11c重组酶（cre）小鼠杂交，培育出了转基因小鼠。与源自Nudt2小鼠的DCs相比，源自这些小鼠的DCs具有更高水平的ApA（约30倍）。有趣的是，DCs中ApA水平的升高使其具有更高的运动性和更低的方向变异性。此外，DCs能够增强免疫保护，这表现为更高的抗原交叉呈递和CD8 OT-I T细胞的致敏。总体而言，该研究表明ApA对DCs的功能有显著影响。Nudt2/CD11c-cre小鼠可作为研究ApA对DCs关键免疫机制影响的有用工具。
