米什米羚（Budorcas taxicolor taxicolor）完整线粒体基因组的测序和特征分析，并与其他羊科物种进行比较。

Sequencing and characterization of the complete mitochondrial genome of Mishmi takin (Budorcas taxicolor taxicolor) and comparison with the other Caprinae species.

机构信息

Wildlife Institute of India, Dehradun, India.

出版信息

Int J Biol Macromol. 2019 Sep 15;137:87-94. doi: 10.1016/j.ijbiomac.2019.06.201. Epub 2019 Jun 26.

DOI:10.1016/j.ijbiomac.2019.06.201

Abstract

Mishmi takin (Budorcas taxicolor taxicolor) is an endangered and least studies species. We sequenced and annotated a 16,584 bp long complete mitogenome of Mishmi takin (B. t. taxicolor). It was encoded by 37 genes: 13 Protein-Coding Genes (PCGs), two ribosomal RNA genes, 22 transfer RNA, and non-coding control region. All tRNA genes show a typical secondary cloverleaf structure, except tRNA in which dihydrouridine arm did not form a stable structure. We observed 82 bp nucleotide deletions in the control region of Mishmi takin. The overall analysis of 13 PCGs, two rRNA and 22 tRNA of Mishmi takin indicates its close relationship with the B. t. tibetana than the B. t. bedfordi. The overall genetic similarity indicated that all takin species is closely related to the P. nayaur than the C. ibex and Ovibos moschatus. This study provides a useful resource for further studies on conservation and population genetics of Budorcas.

摘要

羚牛（Budorcas taxicolor taxicolor）是一种濒危且研究最少的物种。我们对羚牛（B. t. taxicolor）的全长 16584bp 线粒体基因组进行了测序和注释。它由 37 个基因编码：13 个蛋白质编码基因（PCGs）、两个核糖体 RNA 基因、22 个转移 RNA 和非编码控制区。所有 tRNA 基因均表现出典型的四环叶结构，除了 tRNA 中的二氢尿嘧啶臂未形成稳定结构。我们观察到羚牛控制区的 82bp 核苷酸缺失。对 13 个 PCGs、2 个 rRNA 和 22 个 tRNA 的整体分析表明，它与 B. t. tibetana 的关系比与 B. t. bedfordi 的关系更密切。整体遗传相似性表明，所有羚牛物种与 P. nayaur 的关系比与 C. ibex 和 Ovibos moschatus 的关系更密切。本研究为进一步研究羚牛的保护和群体遗传学提供了有用的资源。