College of Animal Sciences and Technology, Sichuan Agricultural University, Chengdu 611130, China.
College of Animal Sciences and Technology, Sichuan Agricultural University, Chengdu 611130, China.
Int J Biol Macromol. 2019 Jan;121:223-232. doi: 10.1016/j.ijbiomac.2018.10.020. Epub 2018 Oct 6.
Takin (Budorcas taxicolor) is one of the most endangered species. However, the taxonomy of takin is still in dispute. Here, the complete mitochondrial genome of Budorcas taxicolor tibetana was reported for the first time, which featured a typical circular molecule of 16,665 bp in length, including 13 PCGs, 22 tRNAs, two rRNAs, and one control region. A + T content was higher than G + C content. All of the genes were encoded on the heavy strand, except for eight tRNAs and ND6 gene. The O region was 49 bp in length and highly conserved in the synthesis and stem-loop regions, and all of the substitutions and indels were found only in the loop structure. Three types of tandem repeat units, six pairs of hairpin loop structure (TACAT, ATGTA) and six CSBs were predicted in the control region. Our results clearly revealed the systematic status of Budorcas species, and the phylogenetic analyses indicated that Budorcas was closer to Capra and Pseudois, rather than to Ovis, which should be merged into the subfamily of Caprinae.
羚牛（Budorcas taxicolor）是最濒危物种之一。然而，羚牛的分类学仍存在争议。在这里，我们首次报道了西藏羚牛（Budorcas taxicolor tibetana）的完整线粒体基因组，其长度为 16665bp 的典型圆形分子，包含 13 个 PCGs、22 个 tRNAs、两个 rRNAs 和一个控制区。A+T 含量高于 G+C 含量。除了 8 个 tRNA 和 ND6 基因外，所有基因都编码在重链上。O 区长 49bp，在合成和茎环区域高度保守，所有的替换和插入都只发生在环结构中。在控制区预测到了三种串联重复单元、六对发夹环结构（TACAT、ATGTA）和六个 CSB。我们的研究结果清楚地揭示了羚牛属的系统地位，系统发育分析表明，羚牛与山羊属和伪雕羚属更近，而不是绵羊属，应归入山羊属。
