Wong D T, Biswas D K
Laboratory of Pharmacology, Harvard School of Dental Medicine, Boston, Massachusetts 02115.
Oncogene. 1987;2(1):67-72.
Repeated local applications of dimethylbenzanthracene (DMBA) induce epidermoid carcinomas in 14 weeks in the cheek pouch of Syrian hamsters. Characteristic histopathological changes similar to the ones observed in human oral cancers can be detected during the development of these tumors in the hamster cheek pouch. Results presented in this report demonstrate that the cellular proto-oncogene c-erbB is amplified and expressed in an epidermoid carcinoma cell line (HCPC-1), derived from one such cheek pouch tumor. The expression of the gene cannot be detected in the untreated cheek pouch tissue whereas the level of expression of the gene is significantly high in the tumor cell line. The expression of c-erbB gene can also be detected in cheek pouch tissue at an early stage (8-9 weeks) of tumor development and in all tumor-bearing tissue of subsequent stages. The stage of expression of c-erbB gene does not coincide with the stage in which extensive hyperplasia of the epithelial cells (5 weeks) is observed. Rather, this coincides with the onset of the early invasion of the connective tissue by the dysplastic epithelium. The expression of c-erbB gene increases in parallel with the increased tumor burden. These results implicate the possible role of c-erbB gene in the genesis of DMBA-induced oral epithelial carcinomas.
在叙利亚仓鼠的颊囊内反复局部应用二甲基苯并蒽（DMBA），14周后可诱发表皮样癌。在仓鼠颊囊内这些肿瘤的发展过程中，可检测到与人类口腔癌中观察到的相似的特征性组织病理学变化。本报告中的结果表明，细胞原癌基因c-erbB在源自此类颊囊肿瘤的表皮样癌细胞系（HCPC-1）中发生扩增并表达。在未处理的颊囊组织中检测不到该基因的表达，而在肿瘤细胞系中该基因的表达水平显著升高。在肿瘤发展的早期阶段（8-9周）以及后续阶段的所有荷瘤组织中也可检测到c-erbB基因的表达。c-erbB基因的表达阶段与观察到上皮细胞广泛增生（5周）的阶段不一致。相反，这与发育异常的上皮细胞开始早期侵袭结缔组织的阶段一致。c-erbB基因的表达随着肿瘤负荷的增加而平行增加。这些结果提示c-erbB基因在DMBA诱导的口腔上皮癌发生过程中可能发挥作用。
