Institute of Cell Biophysics, Russian Acad. Sci., Institutskaya Str. 3, Pushchino, Moscow Region, 142290, Russia.
Institute of Cell Biophysics, Russian Acad. Sci., Institutskaya Str. 3, Pushchino, Moscow Region, 142290, Russia.
Microvasc Res. 2019 Nov;126:103889. doi: 10.1016/j.mvr.2019.103889. Epub 2019 Jun 27.
It is considered that there are six non-overlapping frequency intervals with constant boundaries. These intervals correspond to different mechanisms of skin blood flow regulation. The boundaries do not depend on functional conditions but this statement should be verified. Also it remains unclear how the oscillatory components of skin blood flow are related. Thus the study is aimed to verify statistically the boundaries of frequency intervals, to test the hypothesis that the boundaries depend on age and to search for relationships between spectral components of skin blood flow. The study involved 105 healthy volunteers aged from 20 to 65 years, which were divided into two age groups. Skin blood flow was registered with laser Doppler flowmeter (LDF). Assessments of frequency interval boundaries and relationship between the frequency oscillatory components of blood flow were conducted with histogram approach, bootstrap method and correlation analysis. New frequency interval boundaries were found. They were different in two groups. A linear correlation and frequency areas with moderate (0.5-0.7) and high (>0.7) correlation coefficients were found between spectral components of blood flow. The dependence of these correlations on the age was shown. Thus we proposed a conceptually new approach to analysis of spectral components of skin microhemodynamics and interpretation of results obtained by laser Doppler techniques. This approach is the result of the development of modern understanding of relationships between skin blood flow regulation mechanisms and spectral components of LDF signals. It allows one to have a new look at these relationships as well as demonstrates their dependence on the functional state of the organism as a whole.
人们认为存在六个非重叠的具有恒定边界的频率间隔。这些间隔对应于不同的皮肤血流调节机制。边界不依赖于功能条件，但这一说法尚待验证。此外，皮肤血流的振荡成分之间如何相关也不清楚。因此，本研究旨在通过统计方法验证频率间隔的边界，检验边界依赖于年龄的假设，并寻找皮肤血流的频谱成分之间的关系。该研究纳入了 105 名年龄在 20 至 65 岁之间的健康志愿者，将其分为两个年龄组。使用激光多普勒血流仪（LDF）记录皮肤血流。通过直方图方法、自举法和相关分析评估频率间隔边界和血流频率振荡成分之间的关系。发现了新的频率间隔边界。它们在两组之间有所不同。在血流的频谱成分之间发现了线性相关和中等（0.5-0.7）和高（>0.7）相关系数的频率区域。还显示了这些相关性与年龄的依赖性。因此，我们提出了一种分析皮肤微循环血流频谱成分的新概念方法，并对激光多普勒技术获得的结果进行了解释。这种方法是对皮肤血流调节机制与 LDF 信号频谱成分之间关系的现代理解发展的结果。它使人们能够以新的视角看待这些关系，并展示它们对整个机体功能状态的依赖性。
