Wang Min, Chang Qiaoqiao, Yang Hua, Liu Yongfeng, Wang Chunfang, Hu Fengyun, Wei Hongen, Li Rongshan
Department of Neurology, Shanxi Provincial People's Hospital, Affiliate of Shanxi Medical University, Taiyuan, China.
Shanxi Key Laboratory of Animal and Animal Model of Human Diseases, Taiyuan, China.
Int J Dev Neurosci. 2019 Aug;76:61-64. doi: 10.1016/j.ijdevneu.2019.06.011. Epub 2019 Jun 27.
The BTBR T + Itpr3tf/J (BTBR) mouse has developmental disorders in the central nervous system and many aberrant neuroanatomical structures. However, identification of the pathological mechanisms underlying these abnormal neuroanatomical structures in the brains of BTBR mice is still lacking. Posttranslational modifications (PTMs) are known to be involved in the regulation of diverse cellular processes, and evidence shows that some types of PTMs are associated with the development of the central nervous system. In this study, we detected four novel PTMs in the cerebral cortex of BTBR mice as compared to C57BL/6 J (B6) mice using western blotting. Results revealed that lysine crotonylation and succinylation were elevated in the cerebral cortex of BTBR mice compared to levels in B6 mice. We speculate that elevated profiles of lysine crotonylation and succinylation may be involved in mechanisms related to neuroanatomical abnormalities in cerebral cortex of BTBR mice.
BTBR T + Itpr3tf/J（BTBR）小鼠在中枢神经系统存在发育障碍以及许多异常的神经解剖结构。然而，目前仍缺乏对BTBR小鼠大脑中这些异常神经解剖结构潜在病理机制的鉴定。已知翻译后修饰（PTM）参与多种细胞过程的调控，并且有证据表明某些类型的PTM与中枢神经系统的发育有关。在本研究中，我们通过蛋白质免疫印迹法检测了与C57BL/6 J（B6）小鼠相比，BTBR小鼠大脑皮质中的四种新型PTM。结果显示，与B6小鼠相比，BTBR小鼠大脑皮质中的赖氨酸巴豆酰化和琥珀酰化水平升高。我们推测，赖氨酸巴豆酰化和琥珀酰化水平的升高可能与BTBR小鼠大脑皮质神经解剖异常相关的机制有关。
