Department of Neurology, Shanxi Provincial People's Hospital, Affiliate of Shanxi Medical University, Taiyuan, China.
Department of Medical Imaging, Shanxi Medical University, Taiyuan, China.
Int J Dev Neurosci. 2020 May;80(3):221-233. doi: 10.1002/jdn.10019. Epub 2020 Mar 9.
The BTBR T Itpr3 (BTBR) mouse has developmental disorders in brain and many aberrant neuroanatomical structures and brain dysfunction. However, identification of the pathological mechanisms underlying abnormal brain development in the brains of BTBR mice is still lacking. Increasingly evidence showed that epigenetics plays an important role in the processes of brain development. In this study, we analyzed microRNA (miRNA) and mRNA expression profiles in the cortical brain tissue from BTBR mice, using RNA sequencing. As compared to C57BL/6J (B6) mice, 1,271 differentially expressed genes (DEGs) and 36 known differentially expressed miRNAs (DEMs) were found in the brain from BTBR mice. The functional annotation and categories of DEGs and DEMs were analyzed. Integration analysis identified 103 known miRNA-mRNA interaction pairs. We further verified selected several genes and miRNAs which may be associated with brain development using quantitative RT-PCR (qRT-PCR). Finally, we speculate that reduced myelin-associated oligodendrocytic basic protein and transmembrane proteins 260 may be linked with abnormal brain development in BTBR mice.
BTBR T Itpr3（BTBR）小鼠的大脑发育存在障碍，并且具有许多异常的神经解剖结构和脑功能障碍。然而，BTBR 小鼠大脑中异常脑发育的病理机制仍未确定。越来越多的证据表明，表观遗传学在大脑发育过程中起着重要作用。在这项研究中，我们使用 RNA 测序分析了 BTBR 小鼠皮质脑组织中的 microRNA（miRNA）和 mRNA 表达谱。与 C57BL/6J（B6）小鼠相比，BTBR 小鼠大脑中有 1271 个差异表达基因（DEGs）和 36 个已知的差异表达 miRNA（DEMs）。对 DEGs 和 DEMs 的功能注释和类别进行了分析。整合分析确定了 103 个已知的 miRNA-mRNA 相互作用对。我们进一步使用定量 RT-PCR（qRT-PCR）验证了一些可能与大脑发育相关的选定基因和 miRNA。最后，我们推测髓鞘相关少突胶质细胞碱性蛋白和跨膜蛋白 260 的减少可能与 BTBR 小鼠的异常大脑发育有关。
