二十面体病毒的非对称性。

Asymmetry in icosahedral viruses.

机构信息

Department of Biochemistry and Molecular Biology, Penn State University, W231 Millennium Science Complex, University Park, PA 16802, USA.

Baker Institute for Animal Health, Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.

出版信息

Curr Opin Virol. 2019 Jun;36:67-73. doi: 10.1016/j.coviro.2019.05.006. Epub 2019 Jun 28.

DOI:10.1016/j.coviro.2019.05.006

PMID:31255982

Abstract

Although icosahedral viruses have obvious and highly symmetrical features, asymmetric structural elements are also present. Asymmetric features may be inherent since the genome and location of minor capsid proteins are typically incorporated without adhering to icosahedral symmetry. Asymmetry also develops during the virus life cycle in order to accomplish key functions such as genome packaging, release, and organization. However, resolving asymmetric features complicates image processing during single-particle cryoEM analysis. This review summarizes the current state of knowledge regarding asymmetric structural features with specific examples drawn from members of picornaviridae, parvoviradae, microviradae, and leviviridae.

摘要

尽管二十面体病毒具有明显的高度对称特征，但也存在非对称结构元件。非对称特征可能是固有的，因为基因组和次要衣壳蛋白的位置通常在不遵循二十面体对称性的情况下被纳入。非对称结构也在病毒生命周期中发展，以完成关键功能，如基因组包装、释放和组织。然而，解析非对称特征会使单颗粒 cryoEM 分析中的图像处理变得复杂。本综述总结了目前关于非对称结构特征的知识状态，并以小核糖核酸病毒科、细小病毒科、微病毒科和正粘病毒科的成员为例进行了说明。