Rubes J, Zák M, Horínová Z
Výzkumný ústav veterinárního lékarství, Brno.
Vet Med (Praha). 1987 Nov;32(11):655-8.
The objective of the study was to work out a simple and reliable method of the cytogenetic examination of bovine embryos. Whole embryos free of the zona pellucida, or parts of these embryos, were cultivated for five hours in a medium with 0.05 microgram colcemide per 1 millilitre. The embryos were hypotonized for 10 to 15 minutes in a mixture of medium and distilled water (1:2 ratio) at the temperature of 37 degrees C. Stepwise fixation was used: first in a mixture of acetic acid, methyl alcohol and water (1:4:5), followed by dropping a mixture of acetic acid and methyl alcohol (1:1) onto the embryo already on the microscope slide during microscopic control. The chromosomes were stained with a 3% solution of Giemsa dye for 10 minutes.
该研究的目的是找出一种简单可靠的牛胚胎细胞遗传学检查方法。去除透明带的完整胚胎或这些胚胎的部分组织，在每毫升含0.05微克秋水仙酰胺的培养基中培养5小时。胚胎在37摄氏度下于培养基与蒸馏水的混合液（1:2比例）中低渗处理10至15分钟。采用逐步固定法：首先在乙酸、甲醇和水的混合液（1:4:5）中固定，随后在显微镜观察下将乙酸和甲醇的混合液（1:1）滴加到已置于载玻片上的胚胎上。染色体用3%吉姆萨染液染色10分钟。
