Confer A W, Tabatabai L B, Deyoe B L, Oltjen S L, Hall S M, Oltjen J W, Morton R J, Fulnechek D L, Smith R E, Smith R A
Department of Veterinary Pathology, Oklahoma State University, Stillwater 74078.
Vet Microbiol. 1987 Dec;15(4):325-39. doi: 10.1016/0378-1135(87)90020-4.
Beef heifers were vaccinated on Day 0 with either salt-extractable protein (CSP) or chemically modified CSP (dCSP) from Brucella abortus Strain 19 in Freund's complete adjuvant (FCA). Six weeks later, vaccination was repeated, and heifers received either the homologous or heterologous vaccine. Another group of heifers received only FCA and saline. Vaccinations with CSP or dCSP stimulated marked antibody responses to B. abortus, as detected by standard serologic tests, an enzyme-linked immunosorbent assay, or a quantitative fluorometric immunoassay. Twelve percent of the heifers were seropositive by the CARD test 1 year after vaccination. Vaccination stimulated an increased cell-mediated immune response as measured by lymphocyte blast transformation (LBT) to B. abortus antigens. Fifty-six weeks after the initial vaccination, the heifers were challenged intraconjunctivally with 1.9 X 10(7) colony-forming units of B. abortus strain 2308. Sixty to 83% of the heifers aborted in each group and 70-83% of the heifers were culture positive. There were no significant differences (P greater than 0.05) among groups with respect to the number of abortions or the number of culture-positive heifers. Antibody responses increased rapidly within 4 weeks after challenge. Overall, antibody responses were greater for heifers that aborted than for those that did not abort. These differences were significant (P less than 0.05) only as measured by the fluorometric procedure. The LBT responses appeared to be higher for vaccinates than for the control group, but these differences were not significant (P greater than 0.20). There was a significantly lower (P less than 0.05) LBT response to heat-killed B. abortus in those heifers that aborted compared to those that did not.
在第0天，将肉用小母牛用来自流产布鲁氏菌19菌株的盐可提取蛋白（CSP）或化学修饰的CSP（dCSP）在弗氏完全佐剂（FCA）中进行接种。六周后，重复接种，小母牛接受同源或异源疫苗。另一组小母牛仅接受FCA和生理盐水。通过标准血清学检测、酶联免疫吸附测定或定量荧光免疫测定检测，用CSP或dCSP接种可刺激对流产布鲁氏菌产生明显的抗体反应。接种后1年，通过CARD检测，12%的小母牛血清呈阳性。通过淋巴细胞增殖转化（LBT）测定对流产布鲁氏菌抗原的反应，接种刺激了细胞介导免疫反应增强。初次接种后56周，用1.9×10⁷个流产布鲁氏菌2308菌株的菌落形成单位进行结膜内攻毒。每组60%至83%的小母牛发生流产，70%至83%的小母牛培养呈阳性。在流产数量或培养阳性小母牛数量方面，各组之间无显著差异（P＞0.05）。攻毒后4周内抗体反应迅速增强。总体而言，流产小母牛的抗体反应比未流产的小母牛更强。仅通过荧光测定法测量时，这些差异具有显著性（P＜0.05）。接种疫苗的小母牛的LBT反应似乎高于对照组，但这些差异不具有显著性（P＞0.20）。与未流产的小母牛相比，流产的小母牛对热灭活的流产布鲁氏菌的LBT反应显著更低（P＜0.05）。
